Anti-MAdCAM1 antibody [MECA-367] - Low endotoxin, Azide free (ab185781)

Overview

  • Product name
    Anti-MAdCAM1 antibody [MECA-367] - Low endotoxin, Azide free
    See all MAdCAM1 primary antibodies
  • Description
    Rat monoclonal [MECA-367] to MAdCAM1 - Low endotoxin, Azide free
  • Host species
    Rat
  • Tested applications
    Suitable for: Flow Cyt, Functional Studiesmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control
    • Mouse TNF alpha stimulated bEnd.3 cell line; Mouse bone marrow cells and splenocytes.
  • General notes

    Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.

Properties

Applications

Our Abpromise guarantee covers the use of ab185781 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 105-8 cells.

In a final volume of 100 μL

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Functional Studies Use at an assay dependent concentration.

Target

  • Function
    Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.
  • Tissue specificity
    Highly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ovaries, testis, heart, placenta, lung, liver, skeletal muscle, kidney or peripheral blood leukocytes.
  • Sequence similarities
    Contains 2 Ig-like (immunoglobulin-like) domains.
  • Post-translational
    modifications
    The Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Addressin mucosal antibody
    • hMAdCAM 1 antibody
    • hMAdCAM-1 antibody
    • MACAM1 antibody
    • MADCA_HUMAN antibody
    • MAdCAM 1 antibody
    • MAdCAM-1 antibody
    • Madcam1 antibody
    • Mucosal addressin cell adhesion molecule 1 antibody
    • Mucosal vascular addressin cell adhesion molecule 1 antibody
    see all

References

ab185781 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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