Overview

  • Product nameAnti-MADH7 antibody
    See all MADH7 primary antibodies
  • Description
    Rabbit polyclonal to MADH7
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A synthetic peptide from C terminal residues of human MADH7 (NM_005904).

  • Positive control
    • HeLa cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab90086 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/1000. Predicted molecular weight: 46 kDa.

Target

  • FunctionAntagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
  • Tissue specificityUbiquitous with higher expression in the lung and vascular endothelium.
  • Involvement in diseaseColorectal cancer 3
  • Sequence similaritiesBelongs to the dwarfin/SMAD family.
    Contains 1 MH1 (MAD homology 1) domain.
    Contains 1 MH2 (MAD homology 2) domain.
  • Post-translational
    modifications
    Phosphorylation on Ser-249 does not affect its stability, nuclear localization or inhibitory function in TGFB signaling; however it affects its ability to regulate transcription (By similarity). Phosphorylated by PDPK1.
    Ubiquitinated by WWP1 (By similarity). Polyubiquitinated by RNF111, which is enhanced by AXIN1 and promotes proteasomal degradation. In response to TGF-beta, ubiquitinated by SMURF1; which promotes its degradation.
    Acetylation prevents ubiquitination and degradation mediated by SMURF1.
  • Cellular localizationNucleus. Cytoplasm. Interaction with NEDD4L or RNF111 induces translocation from the nucleus to the cytoplasm (PubMed:16601693). TGF-beta stimulates its translocation from the nucleus to the cytoplasm. PDPK1 inhibits its translocation from the nucleus to the cytoplasm in response to TGF-beta (PubMed:17327236).
  • Information by UniProt
  • Database links
  • Alternative names
    • CRCS3 antibody
    • FLJ16482 antibody
    • hSMAD 7 antibody
    • hSMAD7 antibody
    • MAD (mothers against decapentaplegic Drosophila) homolog 7 antibody
    • MAD antibody
    • Mad homolog 7 antibody
    • MAD homolog 8 antibody
    • MAD mothers against decapentaplegic homolog 7 antibody
    • MADH 7 antibody
    • MADH 8 antibody
    • MADH6 antibody
    • MADH8 antibody
    • Mothers Against Decapentaplegic Drosophila Homolog of 6 antibody
    • Mothers Against Decapentaplegic Drosophila Homolog of 7 antibody
    • Mothers against decapentaplegic homolog 7 antibody
    • Mothers against decapentaplegic homolog 8 antibody
    • Mothers against DPP homolog 7 antibody
    • Mothers against DPP homolog 8 antibody
    • SMA- AND MAD-RELATED PROTEIN 7 antibody
    • SMAD 7 antibody
    • SMAD antibody
    • SMAD family member 7 antibody
    • SMAD, mothers against DPP homolog 7 (Drosophila) antibody
    • SMAD, mothers against DPP homolog 7 antibody
    • SMAD6 antibody
    • Smad7 antibody
    • SMAD7_HUMAN antibody
    see all

Anti-MADH7 antibody images

  • Anti-MADH7 antibody (ab90086) at 1/500 dilution + HeLa cell lysate

    Predicted band size : 46 kDa

References for Anti-MADH7 antibody (ab90086)

This product has been referenced in:
  • Zhang Z  et al. The autoregulatory feedback loop of microRNA-21/programmed cell death protein 4/activation protein-1 (MiR-21/PDCD4/AP-1) as a driving force for hepatic fibrosis development. J Biol Chem 288:37082-93 (2013). WB . Read more (PubMed: 24196965) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"