Anti-MADM antibody - Aminoterminal end (ab37619)

Overview

  • Product nameAnti-MADM antibody - Aminoterminal end
    See all MADM primary antibodies
  • Description
    Rabbit polyclonal to MADM - Aminoterminal end
  • Tested applicationsSuitable for: ICC/IF, IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to N terminal amino acids 1-100 of Human MADM

  • Positive control
    • Human cancer tissue (hepatocarcinoma), mouse brain tissue. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa and HepG2

Properties

Applications

Our Abpromise guarantee covers the use of ab37619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P 1/50 - 1/100.
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).

Target

  • FunctionMay play a role in subcellular trafficking between the endoplasmic reticulum and Golgi apparatus through interactions with the Rho-type GTPases. Binding to the NS3 protein of dengue virus type 2 appears to subvert this activity into the alteration of the intracellular membrane structure associated with flaviviral replication.
  • Tissue specificityUbiquitously expressed in all tissues examined with high levels in the testis.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • DomainThe protein kinase domain is predicted to be catalytically inactive.
  • Cellular localizationCytoplasm > cell cortex. Endomembrane system. Cell projection > lamellipodium. Colocalizes with activated RAC3 to endomembranes and at the cell periphery in lamellipodia.
  • Information by UniProt
  • Database links
  • Alternative names
    • BCON3 antibody
    • MADM antibody
    • MUDPNP antibody
    • Multiple domain putative nuclear protein antibody
    • Myeloid leukemia factor 1 adaptor molecule antibody
    • NRBP antibody
    • NRBP_HUMAN antibody
    • NRBP1 antibody
    • Nuclear receptor binding protein antibody
    • Nuclear receptor-binding protein antibody
    see all

Anti-MADM antibody - Aminoterminal end images

  • ab37619 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab37619 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-MADM antibody - Aminoterminal end (ab37619) at 1/100 dilution

    Lane 1 : HL-60 cell lysate
    Lane 2 : mouse brain tissue lysate

    Secondary
    HRP-anti-rabbit visualised with chemiluminescence.

    Predicted band size : 59 kDa
    Observed band size : 59 kDa
  • ab37619 at 1/50 staining Human cancer tissue (hepatocarcinoma) by IHC-P

References for Anti-MADM antibody - Aminoterminal end (ab37619)

ab37619 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab37619.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"