Anti-MADM antibody - Aminoterminal end (ab37619)

Overview

  • Product name
    Anti-MADM antibody - Aminoterminal end
    See all MADM primary antibodies
  • Description
    Rabbit polyclonal to MADM - Aminoterminal end
  • Tested applications
    Suitable for: ICC/IF, IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to N terminal amino acids 1-100 of Human MADM

  • Positive control
    • Human cancer tissue (hepatocarcinoma), mouse brain tissue. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa and HepG2

Properties

Applications

Our Abpromise guarantee covers the use of ab37619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P 1/50 - 1/100.
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).

Target

  • Function
    May play a role in subcellular trafficking between the endoplasmic reticulum and Golgi apparatus through interactions with the Rho-type GTPases. Binding to the NS3 protein of dengue virus type 2 appears to subvert this activity into the alteration of the intracellular membrane structure associated with flaviviral replication.
  • Tissue specificity
    Ubiquitously expressed in all tissues examined with high levels in the testis.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Domain
    The protein kinase domain is predicted to be catalytically inactive.
  • Cellular localization
    Cytoplasm > cell cortex. Endomembrane system. Cell projection > lamellipodium. Colocalizes with activated RAC3 to endomembranes and at the cell periphery in lamellipodia.
  • Information by UniProt
  • Database links
  • Alternative names
    • BCON3 antibody
    • MADM antibody
    • MUDPNP antibody
    • Multiple domain putative nuclear protein antibody
    • Myeloid leukemia factor 1 adaptor molecule antibody
    • NRBP antibody
    • NRBP_HUMAN antibody
    • NRBP1 antibody
    • Nuclear receptor binding protein antibody
    • Nuclear receptor-binding protein antibody
    see all

Images

  • ab37619 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab37619 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-MADM antibody - Aminoterminal end (ab37619) at 1/100 dilution

    Lane 1 : HL-60 cell lysate
    Lane 2 : mouse brain tissue lysate

    Secondary
    HRP-anti-rabbit visualised with chemiluminescence.

    Predicted band size : 59 kDa
    Observed band size : 59 kDa
  • ab37619 at 1/50 staining Human cancer tissue (hepatocarcinoma) by IHC-P

References

ab37619 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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