Anti-Mael antibody (ab106713)
Key features and details
- Rabbit polyclonal to Mael
- Suitable for: WB, ICC, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Mael antibody -
Description
Rabbit polyclonal to Mael -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
A 16 amino acid peptide near the C terminus of Human Mael (NP_065199).
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Positive control
- Human skeletal muscle tissue; HeLa cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106713 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 49 kDa.
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ICC |
Use a concentration of 5 µg/ml.
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ICC/IF |
Use a concentration of 20 µg/ml.
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 49 kDa. |
ICC
Use a concentration of 5 µg/ml. |
ICC/IF
Use a concentration of 20 µg/ml. |
Target
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Function
Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Its association with piP-bodies suggests a participation in the secondary piRNAs metabolic process. Required for localization of germ-cell factors to the meiotic nuage. -
Tissue specificity
Testis-specific. Expressed in various cancer cell lines, probably due to demethylation of its promoter. -
Sequence similarities
Belongs to the maelstrom family.
Contains 1 HMG box DNA-binding domain. -
Cellular localization
Cytoplasm. Nucleus. Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. Specifically localizes to piP-bodies, a subset of the nuage which contains secondary piRNAs. - Information by UniProt
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Database links
- Entrez Gene: 84944 Human
- Omim: 611368 Human
- SwissProt: Q96JY0 Human
- Unigene: 651245 Human
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Alternative names
- cancer/testis antigen 128 antibody
- CT128 antibody
- FLJ14904 antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab106713 has been referenced in 4 publications.
- Wang Q et al. Morphine suppresses the immune function of lung cancer by up-regulating MAEL expression. BMC Pharmacol Toxicol 23:92 (2022). PubMed: 36476246
- Rocha-da-Silva L et al. Expression of genome defence protein members in proliferating and quiescent rat male germ cells and the Nuage dynamics. PLoS One 14:e0217941 (2019). PubMed: 31181099
- Bothun AM et al. Quantitative Proteomic Profiling of the Human Ovary from Early to Mid-Gestation Reveals Protein Expression Dynamics of Oogenesis and Folliculogenesis. Stem Cells Dev 27:723-735 (2018). PubMed: 29631484
- Cao J et al. High expression of piwi-like RNA-mediated gene silencing 1 is associated with poor prognosis via regulating transforming growth factor-ß receptors and cyclin-dependent kinases in breast cancer. Mol Med Rep 13:2829-35 (2016). PubMed: 26847393