Overview

  • Product nameAnti-Mael antibody
    See all Mael primary antibodies
  • Description
    Rabbit polyclonal to Mael
  • Tested applicationsSuitable for: WB, ICC, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 16 amino acid peptide near the C terminus of Human Mael (NP_065199).

  • Positive control
    • Human skeletal muscle tissue; HeLa cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab106713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 49 kDa.
ICC Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.

Target

  • FunctionPlays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Its association with piP-bodies suggests a participation in the secondary piRNAs metabolic process. Required for localization of germ-cell factors to the meiotic nuage.
  • Tissue specificityTestis-specific. Expressed in various cancer cell lines, probably due to demethylation of its promoter.
  • Sequence similaritiesBelongs to the maelstrom family.
    Contains 1 HMG box DNA-binding domain.
  • Cellular localizationCytoplasm. Nucleus. Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. Specifically localizes to piP-bodies, a subset of the nuage which contains secondary piRNAs.
  • Information by UniProt
  • Database links
  • Alternative names
    • cancer/testis antigen 128 antibody
    • CT128 antibody
    • FLJ14904 antibody
    • mael antibody
    • MAEL_HUMAN antibody
    • maelstrom homolog (Drosophila) antibody
    • Maelstrom homolog antibody
    • OTTHUMP00000032344 antibody
    • Protein maelstrom homolog antibody
    • RP11-102C16.1 antibody
    see all

Anti-Mael antibody images

  • Immunofluorescence of Mael in HeLa cells with ab106713 at 20 ug/mL.

  • Anti-Mael antibody (ab106713) at 1 µg/ml + HeLa cell lysate at 15 µg

    Predicted band size : 49 kDa
  • ab106713, at 5 µg/ml, staining in Hela cells with Maelstrom by Immunocytochemistry.

References for Anti-Mael antibody (ab106713)

ab106713 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
Sample Marmoset (common) Tissue sections (Adult testis)
Specification Adult testis
Permeabilization No
Fixative Formaldehyde
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Submitted Oct 01 2013

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