Overview

  • Product nameAnti-Mael antibody
    See all Mael primary antibodies
  • Description
    Rabbit polyclonal to Mael
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Mouse mMael.

    (Peptide available as ab30463.)

  • Positive control
    • This antibody gave a positive result in Mouse Testis Whole Tissue Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab28661 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
ICC/IF 1/100. PubMed: 18694567

Target

  • FunctionPlays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Its association with piP-bodies suggests a participation in the secondary piRNAs metabolic process. Required for localization of germ-cell factors to the meiotic nuage.
  • Tissue specificityTestis-specific. Expressed in various cancer cell lines, probably due to demethylation of its promoter.
  • Sequence similaritiesBelongs to the maelstrom family.
    Contains 1 HMG box DNA-binding domain.
  • Cellular localizationCytoplasm. Nucleus. Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. Specifically localizes to piP-bodies, a subset of the nuage which contains secondary piRNAs.
  • Information by UniProt
  • Database links
  • Alternative names
    • cancer/testis antigen 128 antibody
    • CT128 antibody
    • FLJ14904 antibody
    • mael antibody
    • MAEL_HUMAN antibody
    • maelstrom homolog (Drosophila) antibody
    • Maelstrom homolog antibody
    • OTTHUMP00000032344 antibody
    • Protein maelstrom homolog antibody
    • RP11-102C16.1 antibody
    see all

Anti-Mael antibody images

  • Anti-Mael antibody (ab28661) at 2 µg/ml + Mouse Testis Whole Tissue Lysate at 20 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size : 49 kDa
    Observed band size : 49 kDa
    Additional bands at : 25 kDa,37 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Mael antibody (ab28661)

This product has been referenced in:
  • Zheng K  et al. Mouse MOV10L1 associates with Piwi proteins and is an essential component of the Piwi-interacting RNA (piRNA) pathway. Proc Natl Acad Sci U S A 107:11841-6 (2010). Read more (PubMed: 20534472) »
  • Ma L  et al. GASZ is essential for male meiosis and suppression of retrotransposon expression in the male germline. PLoS Genet 5:e1000635 (2009). WB ; Mouse . Read more (PubMed: 19730684) »

See all 3 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (various human cell lines)
Loading amount 30 µg
Specification various human cell lines
Gel Running Conditions Non-reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted May 25 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"