Overview

  • Product nameAnti-MAFA antibody
    See all MafA primary antibodies
  • Description
    Rabbit polyclonal to MAFA
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a sequence between C terminal amino acids 285-315 of Human MafA (NP_963883.2).

  • Positive control
    • K-562 cell line lysates

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab124108 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 37 kDa.

Target

  • FunctionActs as a transcriptional factor. Specifically binds the insulin enhancer element RIPE3b and activates insulin gene expression. Cooperates synergistically with NEUROD1 and PDX1. Phosphorylation by GSK3 increases its transcriptional activity and is required for its oncogenic activity. Involved either as an oncogene or as a tumor suppressor, depending on the cell context.
  • Sequence similaritiesBelongs to the bZIP family. Maf subfamily.
    Contains 1 bZIP domain.
  • Post-translational
    modifications
    Ubiquitinated, leading to its degradation by the proteasome.
    Ser-14 and Ser-65 appear to be the major phosphorylation sites. Phosphorylated by MAPK13 on serine and threonine residues (Probable). Phosphorylation by GSK3 requires prior phosphorylation of Ser-65 by another kinase. Phosphorylation proceeds then from Ser-61 to Thr-57, Thr-53 and Ser-49. GSK3-mediated phosphorylation increases its transcriptional activity through the recruitment of the coactivator PCAF, is required for its transforming activity and leads to its degradation through an ubiquitin/proteasome-dependent pathway.
  • Cellular localizationNucleus. Detected in nuclei of pancreas islet beta cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • HMafA antibody
    • Mafa antibody
    • MAFA_HUMAN antibody
    • Pancreatic beta cell specific transcriptional activator antibody
    • Pancreatic beta-cell-specific transcriptional activator antibody
    • RIPE3b1 antibody
    • Transcription factor MafA antibody
    • Transcription factor RIPE3b1 antibody
    • v maf musculoaponeurotic fibrosarcoma oncogene homolog A (avian) antibody
    • v maf musculoaponeurotic fibrosarcoma oncogene homolog A antibody
    • V-maf musculoaponeurotic fibrosarcoma oncogene homolog A antibody
    see all

Anti-MAFA antibody images

  • Anti-MAFA antibody (ab124108) at 1/100 dilution + K-562 cell line lysates at 35 µg

    Predicted band size : 37 kDa

References for Anti-MAFA antibody (ab124108)

ab124108 has not yet been referenced specifically in any publications.

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