Overview

  • Product nameAnti-MAFA antibody
    See all MafA primary antibodies
  • Description
    Mouse monoclonal to MAFA
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: LEERFSDDQL VSMSVRELNR QLRGFSKEEV IRLKQKRRTL KNRGYAQSCR FKRVQQRHIL ESEKCQLQSQ VEQLKLEVGR LAKERDL, corresponding to amino acids 222-309 of Human MAFA

Properties

Applications

Our Abpromise guarantee covers the use of ab57807 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionActs as a transcriptional factor. Specifically binds the insulin enhancer element RIPE3b and activates insulin gene expression. Cooperates synergistically with NEUROD1 and PDX1. Phosphorylation by GSK3 increases its transcriptional activity and is required for its oncogenic activity. Involved either as an oncogene or as a tumor suppressor, depending on the cell context.
    • Sequence similaritiesBelongs to the bZIP family. Maf subfamily.
      Contains 1 bZIP domain.
    • Post-translational
      modifications
      Ubiquitinated, leading to its degradation by the proteasome.
      Ser-14 and Ser-65 appear to be the major phosphorylation sites. Phosphorylated by MAPK13 on serine and threonine residues (Probable). Phosphorylation by GSK3 requires prior phosphorylation of Ser-65 by another kinase. Phosphorylation proceeds then from Ser-61 to Thr-57, Thr-53 and Ser-49. GSK3-mediated phosphorylation increases its transcriptional activity through the recruitment of the coactivator PCAF, is required for its transforming activity and leads to its degradation through an ubiquitin/proteasome-dependent pathway.
    • Cellular localizationNucleus. Detected in nuclei of pancreas islet beta cells.
    • Information by UniProt
    • Database links
    • Alternative names
      • HMafA antibody
      • Mafa antibody
      • MAFA_HUMAN antibody
      • Pancreatic beta cell specific transcriptional activator antibody
      • Pancreatic beta-cell-specific transcriptional activator antibody
      • RIPE3b1 antibody
      • Transcription factor MafA antibody
      • Transcription factor RIPE3b1 antibody
      • v maf musculoaponeurotic fibrosarcoma oncogene homolog A (avian) antibody
      • v maf musculoaponeurotic fibrosarcoma oncogene homolog A antibody
      • V-maf musculoaponeurotic fibrosarcoma oncogene homolog A antibody
      see all

    Anti-MAFA antibody images

    • Western blot against tagged recombinant protein immunogen using ab57807 MAFA antibody at 1ug/ml. Predicted band size of immunogen is 36 kDa

    References for Anti-MAFA antibody (ab57807)

    ab57807 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"