Anti-MAFA antibody (ab85008)
Key features and details
- Rabbit polyclonal to MAFA
- Suitable for: IHC-P
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-MAFA antibody
See all MafA primary antibodies -
Description
Rabbit polyclonal to MAFA -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide corresponding to Mouse MafA aa 300-400 (C terminal).
Database link: Q8CF90 -
Positive control
- Mouse Pancreas tissue.
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General notes
Concentration is optimized for IHC and not determined
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab85008 was affinity purified using the peptide immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85008 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P | (1) |
Notes |
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Likely to work with frozen sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Acts as a transcriptional factor. Specifically binds the insulin enhancer element RIPE3b and activates insulin gene expression. Cooperates synergistically with NEUROD1 and PDX1. Phosphorylation by GSK3 increases its transcriptional activity and is required for its oncogenic activity. Involved either as an oncogene or as a tumor suppressor, depending on the cell context. -
Sequence similarities
Belongs to the bZIP family. Maf subfamily.
Contains 1 bZIP domain. -
Post-translational
modificationsUbiquitinated, leading to its degradation by the proteasome.
Ser-14 and Ser-65 appear to be the major phosphorylation sites. Phosphorylated by MAPK13 on serine and threonine residues (Probable). Phosphorylation by GSK3 requires prior phosphorylation of Ser-65 by another kinase. Phosphorylation proceeds then from Ser-61 to Thr-57, Thr-53 and Ser-49. GSK3-mediated phosphorylation increases its transcriptional activity through the recruitment of the coactivator PCAF, is required for its transforming activity and leads to its degradation through an ubiquitin/proteasome-dependent pathway. -
Cellular localization
Nucleus. Detected in nuclei of pancreas islet beta cells. - Information by UniProt
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Database links
- Entrez Gene: 378435 Mouse
- SwissProt: Q8CF90 Mouse
- Unigene: 309589 Mouse
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Alternative names
- HMafA antibody
- Mafa antibody
- MAFA_HUMAN antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab85008 has not yet been referenced specifically in any publications.