Overview

  • Product nameAnti-MAFA antibody
    See all MafA primary antibodies
  • Description
    Rabbit polyclonal to MAFA
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide, corresponding to a region between C terminal amino acids 300-359 of Mouse MafA (NP_919331.1).

  • Positive control
    • Mouse Pancreas tissue.
  • General notes

    Concentration is optimized for IHC and not determined

Properties

Applications

Our Abpromise guarantee covers the use of ab85008 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notesIHC-P: 1/100 - 1/500. Epitope exposure with citrate buffer or tris-EDTA pH9 buffer will enhance staining. Extended incubation with primary antibody (16-24 hours) at room temperature is recommended.

    Likely to work with frozen sections.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionActs as a transcriptional factor. Specifically binds the insulin enhancer element RIPE3b and activates insulin gene expression. Cooperates synergistically with NEUROD1 and PDX1. Phosphorylation by GSK3 increases its transcriptional activity and is required for its oncogenic activity. Involved either as an oncogene or as a tumor suppressor, depending on the cell context.
    • Sequence similaritiesBelongs to the bZIP family. Maf subfamily.
      Contains 1 bZIP domain.
    • Post-translational
      modifications
      Ubiquitinated, leading to its degradation by the proteasome.
      Ser-14 and Ser-65 appear to be the major phosphorylation sites. Phosphorylated by MAPK13 on serine and threonine residues (Probable). Phosphorylation by GSK3 requires prior phosphorylation of Ser-65 by another kinase. Phosphorylation proceeds then from Ser-61 to Thr-57, Thr-53 and Ser-49. GSK3-mediated phosphorylation increases its transcriptional activity through the recruitment of the coactivator PCAF, is required for its transforming activity and leads to its degradation through an ubiquitin/proteasome-dependent pathway.
    • Cellular localizationNucleus. Detected in nuclei of pancreas islet beta cells.
    • Information by UniProt
    • Database links
    • Alternative names
      • HMafA antibody
      • Mafa antibody
      • MAFA_HUMAN antibody
      • Pancreatic beta cell specific transcriptional activator antibody
      • Pancreatic beta-cell-specific transcriptional activator antibody
      • RIPE3b1 antibody
      • Transcription factor MafA antibody
      • Transcription factor RIPE3b1 antibody
      • v maf musculoaponeurotic fibrosarcoma oncogene homolog A (avian) antibody
      • v maf musculoaponeurotic fibrosarcoma oncogene homolog A antibody
      • V-maf musculoaponeurotic fibrosarcoma oncogene homolog A antibody
      see all

    Anti-MAFA antibody images

    • ab85008 at 1/250 dilution staining MafA in mouse pancreatic islet by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.

    References for Anti-MAFA antibody (ab85008)

    ab85008 has not yet been referenced specifically in any publications.

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