Overview

  • Product nameAnti-MAFA antibody
    See all MafA primary antibodies
  • Description
    Rabbit polyclonal to MAFA
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    3 synthetic peptides (Human) conjugated to KLH

  • Positive control
    • Recombinant protein produced in an induced culture of E.coli.

Properties

Applications

Our Abpromise guarantee covers the use of ab86020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 37 kDa.

Target

  • FunctionActs as a transcriptional factor. Specifically binds the insulin enhancer element RIPE3b and activates insulin gene expression. Cooperates synergistically with NEUROD1 and PDX1. Phosphorylation by GSK3 increases its transcriptional activity and is required for its oncogenic activity. Involved either as an oncogene or as a tumor suppressor, depending on the cell context.
  • Sequence similaritiesBelongs to the bZIP family. Maf subfamily.
    Contains 1 bZIP domain.
  • Post-translational
    modifications
    Ubiquitinated, leading to its degradation by the proteasome.
    Ser-14 and Ser-65 appear to be the major phosphorylation sites. Phosphorylated by MAPK13 on serine and threonine residues (Probable). Phosphorylation by GSK3 requires prior phosphorylation of Ser-65 by another kinase. Phosphorylation proceeds then from Ser-61 to Thr-57, Thr-53 and Ser-49. GSK3-mediated phosphorylation increases its transcriptional activity through the recruitment of the coactivator PCAF, is required for its transforming activity and leads to its degradation through an ubiquitin/proteasome-dependent pathway.
  • Cellular localizationNucleus. Detected in nuclei of pancreas islet beta cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • HMafA antibody
    • Mafa antibody
    • MAFA_HUMAN antibody
    • Pancreatic beta cell specific transcriptional activator antibody
    • Pancreatic beta-cell-specific transcriptional activator antibody
    • RIPE3b1 antibody
    • Transcription factor MafA antibody
    • Transcription factor RIPE3b1 antibody
    • v maf musculoaponeurotic fibrosarcoma oncogene homolog A (avian) antibody
    • v maf musculoaponeurotic fibrosarcoma oncogene homolog A antibody
    • V-maf musculoaponeurotic fibrosarcoma oncogene homolog A antibody
    see all

Anti-MAFA antibody images

  • All lanes : Anti-MAFA antibody (ab86020) at 1/1000 dilution

    Lane 1 : Uninduced (negative control) culture of E.coli
    Lane 2 : Induced culture of E.coli

    Secondary
    anti-Rabbit IgG HRP conjugated at 1/1000 dilution

    Predicted band size : 37 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)

References for Anti-MAFA antibody (ab86020)

ab86020 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab86020.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"