Overview

  • Product nameAnti-MafB antibody
    See all MafB primary antibodies
  • Description
    Rabbit polyclonal to MafB
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Chicken, Non Human Primates
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 MafB.

    (Peptide available as ab71662.)

  • Positive control
    • This antibody gave a positive signal in the following lysates: HeLa Whole Cell, HepG2 Whole Cell, Caco 2 Whole Cell, Rat Thymus Tissue (data not shown), Rat Colon Tissue (data not shown)

Properties

Applications

Our Abpromise guarantee covers the use of ab65953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 36 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionActs as a transcriptional activator or repressor. Plays a pivotal role in regulating lineage-specific hematopoiesis by repressing ETS1-mediated transcription of erythroid-specific genes in myeloid cells. Required for monocytic, macrophage, podocyte and islet beta cell differentiation. Involved in renal tubule survival and F4/80 maturation. Activates the insulin and glucagon promoters. Together with PAX6, transactivates weakly the glucagon gene promoter through the G1 element. SUMO modification controls its transcriptional activity and ability to specify macrophage fate. Binds element G1 on the glucagon promoter (By similarity). Involved either as an oncogene or as a tumor suppressor, depending on the cell context.
  • Tissue specificityUbiquitous.
  • Involvement in diseaseDefects in MAFB are the cause of multicentric carpotarsal osteolysis syndrome (MCTO) [MIM:166300]. MCTO is a rare skeletal disorder, usually presenting in early childhood with a clinical picture mimicking juvenile rheumatoid arthritis. Progressive destruction of the carpal and tarsal bone usually occurs and other bones may also be involved. Chronic renal failure is a frequent component of the syndrome. Mental retardation and minor facial anomalies have been noted in some patients.
  • Sequence similaritiesBelongs to the bZIP family. Maf subfamily.
    Contains 1 bZIP domain.
  • DomainThe leucine-zipper domain is involved in the interaction with LRPICD.
  • Post-translational
    modifications
    Phosphorylated by GSK3 and MAPK13 on serine and threonine residues.
    Sumoylated. Sumoylation on Lys-32 and Lys-297 stimulates its transcriptional repression activity and promotes macrophage differentiation from myeloid progenitors.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Kreisler antibody
    • Kreisler (mouse) maf related leucine zipper homolog antibody
    • Kreisler maf related leucine zipper homolog antibody
    • KRML antibody
    • Maf-B antibody
    • Mafb antibody
    • MAFB/Kreisler basic region/leucine zipper transcription factor antibody
    • MAFB_HUMAN antibody
    • MGC43127 antibody
    • Segmentation protein KR antibody
    • Transcription factor MafB antibody
    • V maf musculoaponeurotic fibrosarcoma oncogene homolog B (avian) antibody
    • V-maf musculoaponeurotic fibrosarcoma oncogene homolog B antibody
    see all

Anti-MafB antibody images

  • All lanes : Anti-MafB antibody (ab65953) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 36 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 105 kDa,55 kDa,63 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab65953 stained MCF-7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65953, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, Hek293, HepG2 cells at 5µg/ml.

References for Anti-MafB antibody (ab65953)

ab65953 has not yet been referenced specifically in any publications.

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