Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab66506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 20436469
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).Can be blocked with MafB peptide (ab205727). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
Flow Cyt Use 4µg for 106 cells.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration.

Target

  • Function
    Acts as a transcriptional activator or repressor. Plays a pivotal role in regulating lineage-specific hematopoiesis by repressing ETS1-mediated transcription of erythroid-specific genes in myeloid cells. Required for monocytic, macrophage, podocyte and islet beta cell differentiation. Involved in renal tubule survival and F4/80 maturation. Activates the insulin and glucagon promoters. Together with PAX6, transactivates weakly the glucagon gene promoter through the G1 element. SUMO modification controls its transcriptional activity and ability to specify macrophage fate. Binds element G1 on the glucagon promoter (By similarity). Involved either as an oncogene or as a tumor suppressor, depending on the cell context.
  • Tissue specificity
    Ubiquitous.
  • Involvement in disease
    Defects in MAFB are the cause of multicentric carpotarsal osteolysis syndrome (MCTO) [MIM:166300]. MCTO is a rare skeletal disorder, usually presenting in early childhood with a clinical picture mimicking juvenile rheumatoid arthritis. Progressive destruction of the carpal and tarsal bone usually occurs and other bones may also be involved. Chronic renal failure is a frequent component of the syndrome. Mental retardation and minor facial anomalies have been noted in some patients.
  • Sequence similarities
    Belongs to the bZIP family. Maf subfamily.
    Contains 1 bZIP domain.
  • Domain
    The leucine-zipper domain is involved in the interaction with LRPICD.
  • Post-translational
    modifications
    Phosphorylated by GSK3 and MAPK13 on serine and threonine residues.
    Sumoylated. Sumoylation on Lys-32 and Lys-297 stimulates its transcriptional repression activity and promotes macrophage differentiation from myeloid progenitors.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Kreisler antibody
    • Kreisler (mouse) maf related leucine zipper homolog antibody
    • Kreisler maf related leucine zipper homolog antibody
    • KRML antibody
    • MAF bZIP transcription factor B antibody
    • Maf-B antibody
    • Mafb antibody
    • MAFB/Kreisler basic region/leucine zipper transcription factor antibody
    • MAFB_HUMAN antibody
    • MGC43127 antibody
    • Segmentation protein KR antibody
    • Transcription factor MafB antibody
    • V maf musculoaponeurotic fibrosarcoma oncogene homolog B (avian) antibody
    • V-maf musculoaponeurotic fibrosarcoma oncogene homolog B antibody
    see all

Images

  • Anti-MafB antibody (ab66506) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 36 kDa
    Observed band size : 36 kDa
    Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analyisis of human kidney tissue labelling MafB with ab66506 at an 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

  • ab66506, at 4 µg/cells, staining MAFB in mouse bone marrow by flow cytometry. Cells were paraformaldehyde fixed and permeabilized using Saponin. The secondary antibody was ab6717, a FITC-conjugated goat polyclonal to rabbit IgG.

    See Abreview

References

This product has been referenced in:
  • Chen J  et al. A Convenient Cas9-based Conditional Knockout Strategy for Simultaneously Targeting Multiple Genes in Mouse. Sci Rep 7:517 (2017). WB . Read more (PubMed: 28364122) »
  • Bunaciu RP  et al. 6-Formylindolo(3,2-b)Carbazole (FICZ) Modulates the Signalsome Responsible for RA-Induced Differentiation of HL-60 Myeloblastic Leukemia Cells. PLoS One 10:e0135668 (2015). WB ; Human . Read more (PubMed: 26287494) »

See all 6 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Bone marrow)
Specification
Bone marrow
Fixative
Paraformaldehyde
Permeabilization
Yes - Saponin
Username

Dr. Kay Muthu

Verified customer

Submitted Jul 16 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Mouse Cell (Bone marrow)
Specification
Bone marrow
Preparation
Cell harvesting/tissue preparation method: Magnetic cell sorting
Sample buffer: IMDM
Fixation
Paraformaldehyde
Permeabilization
Yes - Saponin
Gating Strategy
live cells
Username

Dr. Kay Muthu

Verified customer

Submitted Jul 15 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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