Our Abpromise guarantee covers the use of ab82837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesELISA: Use at an assay dependent dilution.
    Antibody specificity was verified by direct ELISA against the 3 immunogen peptides. A minimum titer of 1/20000 is determined for one of the three peptides.
    WB: 1/500. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionActs as a transcriptional activator or repressor. Plays a pivotal role in regulating lineage-specific hematopoiesis by repressing ETS1-mediated transcription of erythroid-specific genes in myeloid cells. Required for monocytic, macrophage, podocyte and islet beta cell differentiation. Involved in renal tubule survival and F4/80 maturation. Activates the insulin and glucagon promoters. Together with PAX6, transactivates weakly the glucagon gene promoter through the G1 element. SUMO modification controls its transcriptional activity and ability to specify macrophage fate. Binds element G1 on the glucagon promoter (By similarity). Involved either as an oncogene or as a tumor suppressor, depending on the cell context.
    • Tissue specificityUbiquitous.
    • Involvement in diseaseDefects in MAFB are the cause of multicentric carpotarsal osteolysis syndrome (MCTO) [MIM:166300]. MCTO is a rare skeletal disorder, usually presenting in early childhood with a clinical picture mimicking juvenile rheumatoid arthritis. Progressive destruction of the carpal and tarsal bone usually occurs and other bones may also be involved. Chronic renal failure is a frequent component of the syndrome. Mental retardation and minor facial anomalies have been noted in some patients.
    • Sequence similaritiesBelongs to the bZIP family. Maf subfamily.
      Contains 1 bZIP domain.
    • DomainThe leucine-zipper domain is involved in the interaction with LRPICD.
    • Post-translational
      Phosphorylated by GSK3 and MAPK13 on serine and threonine residues.
      Sumoylated. Sumoylation on Lys-32 and Lys-297 stimulates its transcriptional repression activity and promotes macrophage differentiation from myeloid progenitors.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Kreisler antibody
      • Kreisler (mouse) maf related leucine zipper homolog antibody
      • Kreisler maf related leucine zipper homolog antibody
      • KRML antibody
      • Maf-B antibody
      • Mafb antibody
      • MAFB/Kreisler basic region/leucine zipper transcription factor antibody
      • MAFB_HUMAN antibody
      • MGC43127 antibody
      • Segmentation protein KR antibody
      • Transcription factor MafB antibody
      • V maf musculoaponeurotic fibrosarcoma oncogene homolog B (avian) antibody
      • V-maf musculoaponeurotic fibrosarcoma oncogene homolog B antibody
      see all

    Anti-MafB antibody images

    • All lanes : Anti-MafB antibody (ab82837) at 1/500 dilution

      Lane 1 : uninduced culture of E.coli (one shot Top10 competent cells)
      Lane 2 : induced culture of E.coli (one shot Top10 competent cells)

      anti-Rabbit IgG HRP at 1/1000 dilution

      Predicted band size : 50 kDa
      Observed band size : 50 kDa

    References for Anti-MafB antibody (ab82837)

    ab82837 has not yet been referenced specifically in any publications.

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