Overview

  • Product name
  • Description
    Rabbit polyclonal to MAFG
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-162 of Human MAFG (Uniprot ID: O15525).

  • Positive control
    • HeLa cell lysate; Mouse and Rat muscle tissue lysates; HeLa cells; Human PC9 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab154318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 18 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor. Transcription factor, component of erythroid-specific transcription factor NF-E2. Activates globin gene expression when associated with NF-E2. May be involved in signal transduction of extracellular H(+).
  • Tissue specificity
    Highly expressed in skeletal muscle. Also expressed in heart and brain.
  • Sequence similarities
    Belongs to the bZIP family. Maf subfamily.
    Contains 1 bZIP domain.
  • Post-translational
    modifications
    Acetylated in erythroid cells by CREB-binding protein (CBP). Acetylation augments the DNA-binding activity of NFE2, but has no effect on binding NFE2.
    Sumoylation at Lys-14 is required for active transcriptional repression.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AA545192 antibody
    • Basic leucine zipper transcription factor MafG antibody
    • C630022N07Rik antibody
    • hMAF antibody
    • MAFG antibody
    • MAFG v maf musculoaponeurotic fibrosarcoma oncogene homolog G (avian) antibody
    • MAFG_HUMAN antibody
    • MGC13090 antibody
    • MGC20149 antibody
    • RP23 84C12.9 antibody
    • Transcription factor MafG antibody
    • V maf musculoaponeurotic fibrosarcoma oncogene homolog G antibody
    • V-maf musculoaponeurotic fibrosarcoma oncogene homolog G antibody
    see all

Images

  • Anti-MAFG antibody (ab154318) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size : 18 kDa
  • Anti-MAFG antibody (ab154318) at 1/1000 dilution + Mouse muscle tissue lysate at 50 µg

    Predicted band size : 18 kDa
  • Anti-MAFG antibody (ab154318) at 1/1000 dilution + Rat muscle tissue lysate at 50 µg

    Predicted band size : 18 kDa
  • Immunohistochemical analysis of paraffin-embedded Human PC9 xenograft tissue labeling MAFG with ab154318 at 1/500 dilution.
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling MAFG with ab154318 at 1/200 dilution. Right image shows cells co-stained with Hoechst 33342.

References

ab154318 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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