The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use 4 - 8 µl immunoprecipitates MAFF from the A431 cell line.
WB: 1/4000 - 1/8000. Predicted molecular weight: 17 kDa. Dilute the antibody in PBS containing 5.0% non-fat dry milk and 0.05 % Tween 20.
Staining of the MAFF band in immunoblotting is specifically inhibited by the immunizing peptide.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor.
Belongs to the bZIP family. Maf subfamily. Contains 1 bZIP domain.
V maf musculoaponeurotic fibrosarcoma oncogene homolog K (avian) antibody
ChIP - Anti-MAFK antibody (ab50322)This image is courtesy of an anonymous Abreview
ChIP analysis using ab50322 binding MAFK in Human primary macrophage. Cells were cross-linked with formaldehyde. Samples were incubated with primary antibody (1/500) for 12 hours at 4°C. Protein binding was detected using real-time PCR.
Zhang Z et al. Oxidative stress regulates CFTR gene expression in human airway epithelial cells through a distal antioxidant response element. Am J Respir Cell Mol Biol52:387-96 (2015).
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Zheng S et al. TAM receptor-dependent regulation of SOCS3 and MAPKs contributes to proinflammatory cytokine downregulation following chronic NOD2 stimulation of human macrophages. J Immunol194:1928-37 (2015).
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