Overview

  • Product name
  • Description
    Mouse monoclonal to MAG
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Cow, Cat, Human, Monkey
    Predicted to work with: Orangutan
  • Immunogen

    Recombinant fragment: GDLGGYNQYT FSEHSVLDIV NTPNIVVPPE VVAGTEVEVS CMVPDNCPEL RPELSWLGHE GLGEPAVLGR LREDEGTWVQ VSLLHFVPTR, corresponding to amino acids 119-208 of Human MAG (NP_002352) with a proprietary tag.

  • Positive control
    • Partial tagged recombinant Human MAG protein (Immunogen), Jurkat cell lysate. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: SKNSH.

Properties

Applications

Our Abpromise guarantee covers the use of ab89780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/400.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P 1/2000 - 1/4000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 63 kDa. Isoform a, 69 kDa; Isoform b, 63 kDa.
ELISA Use at an assay dependent concentration.

Target

  • Function
    Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha-2,3-linked sialic acid.
  • Sequence similarities
    Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
    Contains 4 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • GMA antibody
    • MAG antibody
    • MAG_HUMAN antibody
    • Myelin associated glycoprotein antibody
    • Myelin-associated glycoprotein antibody
    • S MAG antibody
    • S-MAG antibody
    • Sialic acid binding Ig like lectin 4A antibody
    • Sialic acid binding immunoglobulin like lectin 4A antibody
    • Siglec 4a antibody
    • Siglec-4a antibody
    • SIGLEC4A antibody
    • SPG75 antibody
    see all

Anti-MAG antibody images

  • Anti-MAG antibody (ab89780) at 5 µg/ml + Jurkat cell lysate at 50 µg

    Predicted band size : 63 kDa
    Observed band size : 53 kDa (why is the actual band size different from the predicted?)
  • ab89780 staining MAG in lamb spinal cord tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/5000 in blocking buffer) for 2 hours at 21°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Overlay histogram showing SH-SY5Y cells stained with ab89780 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89780, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min) used under the same conditions.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • ab89780 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab89780 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab89780 staining MAG in Mouse brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with acetone, permeablized with methanol and blocked with 5% BSA for 1 hour at 37°C. The sample was incubated with primary antibody (1/100) at 4°C for 18 hours. An Alexa Fluor® 594-conjugated Goat anti-mouse polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • IHC-P image of MAG staining on cow peripheral nerve sections using ab89780 (1:4000). The sections were fixed in Formaldehyde and subjected to heat mediated antigen retrieval using citric acid (pH 6). The sections were blocked using 1% BSA for 10 mins at 21°C.

    See Abreview

  • Anti-MAG antibody (ab89780) at 5 µg/ml + Partial tagged recombinant Human MAG protein at 0.2 µg

    Predicted band size : 63 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
    Western blot against tagged recombinant protein immunogen. Predicted band size of immunogen is 36 kDa.

References for Anti-MAG antibody (ab89780)

This product has been referenced in:
  • Zia MT  et al. Postnatal glucocorticoid-induced hypomyelination, gliosis, and neurologic deficits are dose-dependent, preparation-specific, and reversible. Exp Neurol 263:200-13 (2015). IHC-Fr ; Rabbit . Read more (PubMed: 25263581) »
  • Wahl SE  et al. Mammalian target of rapamycin promotes oligodendrocyte differentiation, initiation and extent of CNS myelination. J Neurosci 34:4453-65 (2014). Mouse . Read more (PubMed: 24671992) »

See all 9 Publications for this product

Product Wall

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We do not know whether this antibody will recognize S- and/or L-MAG as this has not been specifically tested in our lab. I don't know much about the differences between S- and L-MAG, but in theory if they contain...

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Material Safety Data Sheet can be found on the datasheets of our products.

The datasheet of ab89780 does not include a Material Safety Data Sheet since there are no regulated chemicals in this product. Read More

Thank you for contacting us.
You will be receiving the antibodies at 4C in liquid form. Take out 10 ul of antibody in separate vial and use in experiment. This volume could be higher or lower depending on the use.
To the rest 90ul add 90ul (1...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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