Anti-MAG antibody [EP971Y] (ab46803)

Overview

  • Product nameAnti-MAG antibody [EP971Y]
    See all MAG primary antibodies
  • Description
    Rabbit monoclonal [EP971Y] to MAG
  • Tested applicationsSuitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues near the N-terminus of human MAG.

  • Positive control
    • Rat brain lysate PC-12 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab46803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 100 kDa (predicted molecular weight: 69 kDa).
IP 1/50.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notesIs unsuitable for ICC/IF or IHC-P.
  • Target

    • FunctionAdhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha-2,3-linked sialic acid.
    • Sequence similaritiesBelongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
      Contains 4 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • GMA antibody
      • MAG antibody
      • MAG_HUMAN antibody
      • Myelin associated glycoprotein antibody
      • Myelin-associated glycoprotein antibody
      • S MAG antibody
      • S-MAG antibody
      • Sialic acid binding Ig like lectin 4A antibody
      • Sialic acid binding immunoglobulin like lectin 4A antibody
      • Siglec 4a antibody
      • Siglec-4a antibody
      • SIGLEC4A antibody
      • SPG75 antibody
      see all

    Anti-MAG antibody [EP971Y] images

    • Anti-MAG antibody [EP971Y] (ab46803) at 1/5000 dilution + Rat brain lysate at 10ug/lane

      Secondary
      Goat-anti rabbit, labelled with HRP. at 1/2000 dilution

      Predicted band size : 69 kDa
      Observed band size : 100 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 22 kDa (possible non-specific binding).

    References for Anti-MAG antibody [EP971Y] (ab46803)

    ab46803 has not yet been referenced specifically in any publications.

    Product Wall

    DISCOUNT CODE: 100%ABR-EXXXXX
    Expiration date: 27/01/2013
    I am very pleased to hear you would like to accept our offer and test ab46803 in IHC-PFA fixed mouse brain. This code will give you: 1 free PRIMARY ANTIBODY/PROTEIN OR VALUE OFF OF ORD...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"