Overview

  • Product name
  • Description
    Rabbit polyclonal to MAG1
  • Specificity
    Detects endogenous levels of total PLCH protein.
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human MAG1 aa 381-430 (C terminal).
    Database link: Q53EU6

  • Positive control
    • Extracts from Jurkat, HeLa, and COLO205 cells. HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab64979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
ELISA 1/1000.
ICC/IF 1/100 - 1/500.

Target

  • Function
    Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis. Overexpression activates the mTOR pathway.
  • Tissue specificity
    Widely expressed. Expressed in liver, kidney, testis, brain, heart, skeletal muscle, thyroid, prostate, thymus and placenta. Also expressed lung and adipose tissue.
  • Pathway
    Glycerolipid metabolism; triacylglycerol biosynthesis.
    Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3.
  • Sequence similarities
    Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.
  • Domain
    The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1 acylglycerol 3 phosphate O acyltransferase 8 antibody
    • 1 acylglycerol 3 phosphate O acyltransferase 9 antibody
    • 1 AGP acyltransferase 9 antibody
    • 1 AGPAT 9 antibody
    • 1-acylglycerol-3-phosphate O-acyltransferase 9 antibody
    • 1-AGP acyltransferase 9 antibody
    • 1-AGPAT 9 antibody
    • Acyl CoA:glycerol 3 phosphate acyltransferase 3 antibody
    • Acyl-CoA:glycerol-3-phosphate acyltransferase 3 antibody
    • AGPAT8 antibody
    • AGPAT9 antibody
    • Endoplasmic reticulum associated GPAT antibody
    • Glycerol-3-phosphate acyltransferase 3 antibody
    • GPAT-3 antibody
    • GPAT3 antibody
    • GPAT3_HUMAN antibody
    • hGPAT3 antibody
    • HMFN0839 antibody
    • LPAAT theta antibody
    • LPAAT-theta antibody
    • Lung cancer metastasis associated protein antibody
    • Lung cancer metastasis-associated protein 1 antibody
    • Lysophosphatidic acid acyltransferase theta antibody
    • MAG 1 antibody
    • MAG-1 antibody
    • MGC11324 antibody
    • PLCH antibody
    see all

Anti-MAG1 antibody images

  • This image shows HepG2 cells stained with ab64979 at 1/100 -1/500 dilution; with and without the immunising peptide.

  • All lanes : Anti-MAG1 antibody (ab64979) at 1/500 dilution

    Lane 1 : extracts from Jurkat cells (5-30µg total protein)
    Lane 2 : extracts from HeLa cells (5-30µg total protein)
    Lane 3 : extracts from COLO205 cells (5-30µg total protein)
    Lane 4 : extracts from COLO205 cells (5-30µg total protein) and 5-10µg of the immunising peptide


    Predicted band size : 49 kDa
    Observed band size : 49 kDa
    Additional bands at : 117 kDa,22 kDa. We are unsure as to the identity of these extra bands.

References for Anti-MAG1 antibody (ab64979)

ab64979 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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