Our Abpromise guarantee covers the use of ab21472 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesELISA: Use at a concentration of 0.1 - 1 µg/ml.
    ICC: 1/20.
    ICC/IF: Used at a dilution of 1/25 (see Abreview).
    IHC-P: 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    IP: Use 3µg for 107 cells.
    WB: Use at a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 34 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMay be involved in transcriptional regulation through interaction with SNW1 and recruiting histone deactelyase HDAC1. May inhibit notch intracellular domain (NICD) transactivation. May play a role in embryonal development and tumor transformation or aspects of tumor progression. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.
    • Tissue specificityExpressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes. Never expressed in kidney tumors, leukemias and lymphomas.
    • Sequence similaritiesContains 1 MAGE domain.
    • Cellular localizationCytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Antigen MZ2 E antibody
      • Antigen MZ2-E antibody
      • Cancer/testis antigen 1.1 antibody
      • cancer/testis antigen family 1, member 1 antibody
      • CT1.1 antibody
      • MAGA1_HUMAN antibody
      • MAGE 1 antigen antibody
      • MAGE-1 antigen antibody
      • MAGE1 antibody
      • MAGE1A antibody
      • MAGEA1 antibody
      • Melanoma antigen 1 antibody
      • Melanoma antigen family A 1 (directs expression of antigen MZ2 E) antibody
      • Melanoma antigen family A 1 antibody
      • Melanoma antigen MAGE 1 antibody
      • Melanoma associated antigen 1 antibody
      • Melanoma associated antigen MZ2 E antibody
      • Melanoma-associated antigen 1 antibody
      • MGC9326 antibody
      see all

    Anti-MAGE 1 antibody images

    • Immunohistochemistry of formalin-fixed human melanoma stained for MAGE 1.
    • ab21472 staining the human breast cancer cell line, MCF-7, by ICC/IF. The cell line was stimulated with 5-aza-CdR for 72 hours. The treated cells, or a mock control were then stimulated by trichostatin A for 24 hours. ab21472 was used at a dilution of 1/25 and an Alexa Fluor ® 546 conjugated goat anti-rabbit IgG was used as the secondary.

      See Abreview

    References for Anti-MAGE 1 antibody (ab21472)

    This product has been referenced in:
    • Kawada J  et al. Heteroclitic serological response in esophageal and prostate cancer patients after NY-ESO-1 protein vaccination. Int J Cancer 130:584-92 (2012). ELISA ; Human . Read more (PubMed: 21413013) »
    • Wischnewski F  et al. Promoter demethylation and histone acetylation mediate gene expression of MAGE-A1, -A2, -A3, and -A12 in human cancer cells. Mol Cancer Res 4:339-49 (2006). Read more (PubMed: 16687489) »

    See all 2 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (Bladder cancer cells)
    Loading amount 20 µg
    Specification Bladder cancer cells
    Gel Running Conditions Non-reduced Denaturing (12)
    Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

    Dr. santosh chauhan

    Verified customer

    Submitted Aug 09 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (Breast Cancer Cell Line MCF-7)
    Specification Breast Cancer Cell Line MCF-7
    Fixative Formaldehyde
    Blocking step No blocking step used for 20 minute(s)

    Dr. Heidi Schwarzenbach

    Verified customer

    Submitted Aug 08 2006