Overview

  • Product nameAnti-MAGEA12 antibody
    See all MAGEA12 primary antibodies
  • Description
    Mouse monoclonal to MAGEA12
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment: TLPTTINYTL WSQSDEGSSN EEQEGPSTFP DLETSFQVAL SRKMAELVHF LLLKYRAREP FTKAEMLGSV IRNFQDFFPV IFSKASEYLQ LVFGIEVVE, corresponding to amino acids 70 - 168 of Human MAGEA12 (NP_005358) with a 26 kDa tag

  • Positive control
    • Human liver tissue lysate, Raw 264.7 cell lysate, Jurkat cell lysate and NIH/3T3 cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab87973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa.
ELISA Use at an assay dependent dilution.

Target

  • FunctionNot known, though may play a role in tumor transformation or aspects of tumor progression.
  • Tissue specificityExpressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes.
  • Sequence similaritiesContains 1 MAGE domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cancer/testis antigen 1.12 antibody
    • Cancer/testis antigen family 1 member 12 antibody
    • CT1.12 antibody
    • MAGAC_HUMAN antibody
    • MAGE 12 antigen antibody
    • MAGE-12 antigen antibody
    • MAGE12 antibody
    • MAGE12 antigen antibody
    • MAGE12F antibody
    • MAGE12F antigen antibody
    • MAGEA12 antibody
    • Melanoma antigen family A 12 antibody
    • Melanoma associated antigen 12 antibody
    • Melanoma-associated antigen 12 antibody
    see all

Anti-MAGEA12 antibody images

  • Anti-MAGEA12 antibody (ab87973) at 1 µg/ml + recombinant protein fragment with a proprietary tag (the immunogen) at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 35 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-MAGEA12 antibody (ab87973) at 1 µg/ml + Raw 264.7 cell lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 35 kDa
  • Anti-MAGEA12 antibody (ab87973) at 1 µg/ml + jurkat cell lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 35 kDa
  • Anti-MAGEA12 antibody (ab87973) at 1 µg/ml + human liver tissue lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 35 kDa
  • Anti-MAGEA12 antibody (ab87973) at 1 µg/ml + NIH/3T3 cell lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 35 kDa

References for Anti-MAGEA12 antibody (ab87973)

ab87973 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"