Overview

  • Product nameAnti-MAGEA3 antibody
    See all MAGEA3 primary antibodies
  • Description
    Rabbit polyclonal to MAGEA3
  • Tested applicationsSuitable for: IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    HISYPPLHEWVLREGEE

    conjugated to KLH, corresponding to amino acids 298-314 of Human MAGEA3

  • Positive control
    • Human testis tissue

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab115332 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA 1/1000.

Target

  • FunctionProposed to enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in embryonal development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.
  • Tissue specificityExpressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta. Never expressed in kidney tumors, Leukemias and lymphomas.
  • Sequence similaritiesContains 1 MAGE domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antigen MZ2 D antibody
    • Antigen MZ2-D antibody
    • Cancer/testis antigen 1.3 antibody
    • Cancer/testis antigen family 1 member 3 antibody
    • CT1.3 antibody
    • HIP8 antibody
    • HYPD antibody
    • MAGA3_HUMAN antibody
    • MAGE 3 antigen antibody
    • MAGE-3 antigen antibody
    • MAGE3 antibody
    • MAGEA3 antibody
    • MAGEA6 antibody
    • Melanoma antigen family A 3 antibody
    • Melanoma associated antigen 3 antibody
    • Melanoma-associated antigen 3 antibody
    • MGC1461 antibody
    see all

Anti-MAGEA3 antibody images

  • ab115332 at 10ug/ml staining MAGEA3 in Human testis tissue by immunohistochemistry (FFPE). Following primary incubation slides were incubated with biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References for Anti-MAGEA3 antibody (ab115332)

ab115332 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"