Overview

  • Product name
  • Description
    Rabbit polyclonal to MAGEA3
  • Tested applications
    Suitable for: ELISA, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (10-30 amino acids in length) conjugated to KLH, from the C terminal of the last 50 amino acids of Human MAGEA3.

  • Positive control
    • Jurkat lysate. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: A431.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    This antibody was purified through a protein G column and eluted out with both high and low pH buffers. It was neutralized immediately after elution, followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab38496 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
ICC/IF Use a concentration of 5 µg/ml.
WB 1/100 - 1/500. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).

Target

  • Function
    Proposed to enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in embryonal development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.
  • Tissue specificity
    Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta. Never expressed in kidney tumors, Leukemias and lymphomas.
  • Sequence similarities
    Contains 1 MAGE domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antigen MZ2 D antibody
    • Antigen MZ2-D antibody
    • Cancer/testis antigen 1.3 antibody
    • Cancer/testis antigen family 1 member 3 antibody
    • CT1.3 antibody
    • HIP8 antibody
    • HYPD antibody
    • MAGA3_HUMAN antibody
    • MAGE 3 antigen antibody
    • MAGE family member A3 antibody
    • MAGE-3 antigen antibody
    • MAGE3 antibody
    • MAGEA3 antibody
    • MAGEA6 antibody
    • Melanoma antigen family A 3 antibody
    • Melanoma associated antigen 3 antibody
    • Melanoma-associated antigen 3 antibody
    • MGC1461 antibody
    see all

Images

  • ICC/IF image of ab38496 stained A431 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab38496 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-MAGEA3 antibody (ab38496) at 1/100 dilution + Jurkat lysate

    Predicted band size : 35 kDa
    Observed band size : 35 kDa
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:
  • Junwei W  et al. In vivo enhancement of the MAGE-specific cellular immune response by a recombinant MAGE1-MAGE3-TBHSP70 tumor vaccine. Cancer Cell Int 16:45 (2016). Read more (PubMed: 27330408) »

See 1 Publication for this product

Customer reviews and Q&As

Thanks for your reply. I am sending a free of charge vial of ab63931 on the order ***, which should arrive tomorrow. Please keep me updated of the results using this replacement antibody, and let me know if there is anything else that we can do ...

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Thank you for your reply, and I'm sorry to hear that the antibody still did not work at 1:100. Did you see any bands at all on the blot, even at unexpected molecular weights? I would be happy to send a different antibody as a replacement. What ...

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Thanks for contacting us and letting us know about the trouble with ab38496. What species are the testis, Sertoli, and germ cells from? This antibody is tested in Jurkat cell lysate at a 1:100 dilution, so we would recommend increasing the conce...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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