Anti-MAGEA4 antibody (ab115341)

Overview

  • Product name
  • Description
    Rabbit polyclonal to MAGEA4
  • Tested applications
    Suitable for: IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    GLVGAQAPTTEEQEA

    conjugated to KLH, corresponding to amino acids 24-38 of Human MAGEA4

  • Positive control
    • Human Testis tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab115341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA 1/1000.

Target

  • Function
    Not known, though may play a role in embryonal development and tumor transformation or aspects of tumor progression.
  • Tissue specificity
    Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta.
  • Sequence similarities
    Contains 1 MAGE domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cancer/testis antigen 1.4 antibody
    • CT1.4 antibody
    • MAGA4_HUMAN antibody
    • MAGE 4 antibody
    • MAGE 4 antigen antibody
    • MAGE 41 antibody
    • MAGE 41 antigen antibody
    • MAGE 4A antibody
    • MAGE 4B antibody
    • MAGE X2 antibody
    • MAGE X2 antigen antibody
    • MAGE-4 antigen antibody
    • MAGE-41 antigen antibody
    • MAGE-X2 antigen antibody
    • MAGE4 antibody
    • MAGE41 antibody
    • MAGE4A antibody
    • MAGE4B antibody
    • MAGEA 4 antibody
    • MAGEA4 antibody
    • Melanoma antigen family A 4 antibody
    • Melanoma associated antigen 4 antibody
    • Melanoma-associated antigen 4 antibody
    • MGC21336 antibody
    see all

Images

  • Testis (formalin-fixed, paraffin-embedded) stained with ab115341 at 10 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References

ab115341 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (testes ( 8dpp))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM Sodium Citrate 0.05% Tween-20
Permeabilization
No
Specification
testes ( 8dpp)
Blocking step
Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Formaldehyde
Username

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Verified customer

Submitted Mar 06 2017

Thank you for your enquiry.

We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storage...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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