• Product name
  • Description
    Rabbit polyclonal to MAGEA4
  • Specificity
    While ab76177 has not been tested for cross-reactivity with other MAGE proteins, the immunogen shows >80% sequence homology with MAGEA1, MAGEA2, MAGEA3, MAGEB3, MAGEA6, MAGEA8, MAGEA9, MAGEA10, and MAGEA12. Based on this information, ab76177 may cross-react with these proteins.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    synthetic peptide from the C-terminus of Human MAGEA4

  • Positive control
    • HepG2 cell lysate.



Our Abpromise guarantee covers the use of ab76177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • Function
    Not known, though may play a role in embryonal development and tumor transformation or aspects of tumor progression.
  • Tissue specificity
    Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta.
  • Sequence similarities
    Contains 1 MAGE domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cancer/testis antigen 1.4 antibody
    • CT1.4 antibody
    • MAGA4_HUMAN antibody
    • MAGE 4 antibody
    • MAGE 4 antigen antibody
    • MAGE 41 antibody
    • MAGE 41 antigen antibody
    • MAGE 4A antibody
    • MAGE 4B antibody
    • MAGE X2 antibody
    • MAGE X2 antigen antibody
    • MAGE-4 antigen antibody
    • MAGE-41 antigen antibody
    • MAGE-X2 antigen antibody
    • MAGE4 antibody
    • MAGE41 antibody
    • MAGE4A antibody
    • MAGE4B antibody
    • MAGEA 4 antibody
    • MAGEA4 antibody
    • Melanoma antigen family A 4 antibody
    • Melanoma associated antigen 4 antibody
    • Melanoma-associated antigen 4 antibody
    • MGC21336 antibody
    see all

Anti-MAGEA4 antibody images

  • Anti-MAGEA4 antibody (ab76177) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 35 kDa
    Observed band size : 35 kDa

References for Anti-MAGEA4 antibody (ab76177)

This product has been referenced in:
  • Altman E  et al. Characterization of human spermatogonial stem cell markers in fetal, pediatric, and adult testicular tissues. Reproduction 148:417-27 (2014). Read more (PubMed: 25030892) »

See 1 Publication for this product

Product Wall

Thank you for your reply. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number ***.

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