Anti-MAGEA6 antibody (ab38495)

Overview

  • Product nameAnti-MAGEA6 antibody
    See all MAGEA6 primary antibodies
  • Description
    Rabbit polyclonal to MAGEA6
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Cynomolgus Monkey
  • Immunogen

    Synthetic peptide:

    NYLEYRQVPGSDPAC

    conjugated to KLH, corresponding to Internal sequence amino acids 254-268 of Human MAGEA6.

  • Positive control
    • A549 lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesThis antibody was purified through a protein G column and eluted out with both high and low pH buffers. It was neutralized immediately after elution, followed by dialysis against PBS.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab38495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).

Target

  • FunctionNot known, though may play a role in tumor or aspects of tumor progression.
  • Tissue specificityExpressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes.
  • Sequence similaritiesContains 1 MAGE domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cancer/testis antigen 1.6 antibody
    • Cancer/testis antigen family 1, member 6 antibody
    • CT1.6 antibody
    • MAGA6_HUMAN antibody
    • MAGE 3b antibody
    • MAGE 6 antigen antibody
    • MAGE-6 antigen antibody
    • MAGE3B antibody
    • MAGE3B antigen antibody
    • MAGE6 antibody
    • MAGEA6 antibody
    • Melanoma antigen family A 6 antibody
    • Melanoma associated antigen 6 antibody
    • Melanoma-associated antigen 6 antibody
    see all

Anti-MAGEA6 antibody images

  • Anti-MAGEA6 antibody (ab38495) at 1/100 dilution + A549 lysate

    Predicted band size : 35 kDa
    Observed band size : 35 kDa

References for Anti-MAGEA6 antibody (ab38495)

ab38495 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Human Lung Carcinoma)
Loading amount 30 µg
Specification Human Lung Carcinoma
Gel Running Conditions Reduced Denaturing
Blocking step Tried both 5% milk and 5% BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Username

Dr. Angelique Whitehurst

Verified customer

Submitted Oct 04 2012

Thank you for contacting Abcam earlier today.

I am sorry about the issues you are having with ab38495 in western blot and as I mentioned before the antibody is covered under our Abpromise for six months and is guaranteed to work in western blo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"