Overview

  • Product name
  • Description
    Rabbit polyclonal to MAGEA6
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Cynomolgus monkey
  • Immunogen

    Synthetic peptide:

    NYLEYRQVPGSDPAC

    conjugated to KLH, corresponding to Internal sequence amino acids 254-268 of Human MAGEA6.

  • Positive control
    • A549 lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    This antibody was purified through a protein G column and eluted out with both high and low pH buffers. It was neutralized immediately after elution, followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab38495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).

Target

  • Function
    Not known, though may play a role in tumor or aspects of tumor progression.
  • Tissue specificity
    Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes.
  • Sequence similarities
    Contains 1 MAGE domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cancer/testis antigen 1.6 antibody
    • Cancer/testis antigen family 1, member 6 antibody
    • CT1.6 antibody
    • MAGA6_HUMAN antibody
    • MAGE 3b antibody
    • MAGE 6 antigen antibody
    • MAGE-6 antigen antibody
    • MAGE3B antibody
    • MAGE3B antigen antibody
    • MAGE6 antibody
    • MAGEA6 antibody
    • Melanoma antigen family A 6 antibody
    • Melanoma associated antigen 6 antibody
    • Melanoma-associated antigen 6 antibody
    see all

Images

  • Anti-MAGEA6 antibody (ab38495) at 1/100 dilution + A549 lysate

    Predicted band size : 35 kDa
    Observed band size : 35 kDa

References

This product has been referenced in:
  • Blalock LT  et al. Human dendritic cells adenovirally-engineered to express three defined tumor antigens promote broad adaptive and innate immunity. Oncoimmunology 1:287-357 (2012). Read more (PubMed: 22737604) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (Human Lung Carcinoma)
Loading amount
30 µg
Specification
Human Lung Carcinoma
Gel Running Conditions
Reduced Denaturing
Blocking step
Tried both 5% milk and 5% BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Username

Dr. Angelique Whitehurst

Verified customer

Submitted Oct 04 2012

Thank you for contacting Abcam earlier today.

I am sorry about the issues you are having with ab38495 in western blot and as I mentioned before the antibody is covered under our Abpromise for six months and is guaranteed to work in western blo...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up