Overview

  • Product name
  • Description
    Rabbit polyclonal to MAGEA9
  • Specificity
    This antibody is specific for MAGEA9.
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A KLH conjugated synthetic peptide (10-30 aa in length) in the region of 211~223 of human MAGEA9.

  • Positive control
    • Breast carcinoma tissue and Placental lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab38493 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Detects a band of approximately 40 kDa (predicted molecular weight: 35 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/1000.

Target

  • Function
    Not known, though may play a role in embryonal development and tumor transformation or aspects of tumor progression.
  • Tissue specificity
    Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta.
  • Sequence similarities
    Contains 1 MAGE domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cancer/testis antigen 1.9 antibody
    • Cancer/testis antigen family 1 member 9 antibody
    • CT1.9 antibody
    • MAGA9_HUMAN antibody
    • MAGE 9 antigen antibody
    • MAGE-9 antigen antibody
    • MAGE9 antibody
    • MAGEA9A antibody
    • MAGEA9B antibody
    • Melanoma antigen family A 9 antibody
    • Melanoma associated antigen 9 antibody
    • Melanoma-associated antigen 9 antibody
    • MGC8421 antibody
    see all

Images

  • Anti-MAGEA9 antibody (ab38493) at 1/100 dilution + Placenta lysate

    Predicted band size : 35 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with ab38493 at a 1/50 dilution, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

References

ab38493 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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