• Product name
  • Description
    Rabbit polyclonal to MAGED2
  • Specificity
    This antibody is specific for MAGED2 (C term).
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A KLH conjugated synthetic peptide (10-30 aa in length) at the C-term of last 50 aa of human MAGED2.

  • Positive control
    • Hepatocarcinoma tissue and A549 cell lysate This antibody gave a positive result when used in the following methanol fixed cell lines: PC3



Our Abpromise guarantee covers the use of ab38491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
WB 1/100 - 1/500. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
IHC-P 1/50 - 1/100.
ELISA 1/1000.


  • Relevance
    MAGED2 is a member of the MAGED gene family. In contrast to the MAGEA and MAGEB genes, which have their entire coding sequences located in the last exon, the MAGED genes have their coding sequences located throughout multiple exons. Also, the MAGEA and MAGEB genes are silent in normal tissues with the exception of testis and placenta, whereas the MAGED genes are expressed ubiquitously.
  • Cellular localization
  • Database links
  • Alternative names
    • BCG 1 antibody
    • HCA10 antibody
    • JCL 1 antibody
    • MAGE D antibody
    • MAGE D2 antigen antibody
    • Melanoma antigen family D 2 antibody
    • Melanoma associated antigen D2 antibody
    see all


  • ICC/IF image of ab38491 stained PC3 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab38491 at 1/200 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-MAGED2 antibody (ab38491) + A549 lysate

    Predicted band size : 65 kDa
    Observed band size : 65 kDa
  • Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ab38491 at a dilution of 1/50, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

    This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.


This product has been referenced in:
  • Tseng HY  et al. The melanoma-associated antigen MAGE-D2 suppresses TRAIL receptor 2 and protects against TRAIL-induced apoptosis in human melanoma cells. Carcinogenesis 33:1871-81 (2012). Human . Read more (PubMed: 22791814) »

See 1 Publication for this product

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