Overview

  • Product name
  • Description
    Rabbit polyclonal to MAGEE1
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 413-512 of Human MAGEE1.

  • Positive control
    • Human cerebral cortex tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab121161 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Sequence similarities
    Contains 2 MAGE domains.
  • Cellular localization
    Cytoplasm > perinuclear region. Nucleus. Cell membrane. In the skeletal muscle, found at the postsynaptic membrane and is associated with a subset of myonuclei. May reside within nuclei and/or in perinuclear compartments. In peripheral nerves, co-localizes with DTNA in the Schwann cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI847422 antibody
    • Alpha-dystrobrevin-associated MAGE Protein antibody
    • DAMAGE antibody
    • HCA1 antibody
    • Hepatocellular carcinoma-associated protein 1 antibody
    • MAGE-E1 antigen antibody
    • MAGE1_HUMAN antibody
    • MAGEE1 antibody
    • Melanoma-associated antigen E1 antibody
    • mMage-e1 antibody
    • RGD1560259 antibody
    • RP23-82A12.1 antibody
    • RP4-596C3.1 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in cytoplasm. Recommended concentration of ab121161 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121161 at 1/35 dilution staining MAGEE1 in Paraffin-emebedded Human cerebral cortex tissue by Immunohistochemistry.

    Note: strong cytoplasmic positivity in neuronal cells.

References

ab121161 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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