Anti-MAGEE2 antibody (ab122118)
Key features and details
- Rabbit polyclonal to MAGEE2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MAGEE2 antibody -
Description
Rabbit polyclonal to MAGEE2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: EALKFVSDAH DEEPWSWPEE YNKALEGDKT KERSLTAGLE FWSEDTMNDK ANDLVQLAIS VTEEMLPIHQ DELLAHTGKE FEDVFPNILN RATLILDMFY GLSLIEVDT corresponding to amino acids of Human MAGEE2.
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Positive control
- Human colon tissue; MAGEE2 over-expression HEK293T lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122118 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment |
Notes |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
Target
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Sequence similarities
Contains 2 MAGE domains. - Information by UniProt
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Database links
- Entrez Gene: 139599 Human
- Omim: 300760 Human
- SwissProt: Q8TD90 Human
- Unigene: 356869 Human
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Alternative names
- HCA3 antibody
- Hepatocellular carcinoma-associated protein 3 antibody
- Hepatocellular carcinoma-associated protein HCA32 antibody
see all
Images
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Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus and nucleoli. Recommended concentration of ab122118 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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All lanes : Anti-MAGEE2 antibody (ab122118) at 1/250 dilution
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells
Developed using the ECL technique. -
ab122118, at 1/50 dilution, staining MAGEE2 in paraffin embedded Human colon tissue by Immunohistochemistry.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab122118 has not yet been referenced specifically in any publications.