Overview

  • Product nameAnti-MAGI1 antibody
    See all MAGI1 primary antibodies
  • Description
    Rabbit polyclonal to MAGI1
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A KLH conjugated synthetic peptide (10-30 aa in length) at the C terminus of last 50 aa of Human BAIAP1, based on the mutated sequence in Q96QZ7-3 or 4.

  • Positive control
    • Mouse brain cell lysate and Human hepatocarcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab37543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 137 kDa (predicted molecular weight: 137 kDa).
IHC-P 1/50 - 1/100.

Target

  • RelevanceThe MAGUK (Membrane-Associated Guanylate Kinase) family of proteins is characterized by the presence of multi-PDZ and SH3 domains, and a single region of homology to Saccharomyces cerevisiae guanylate kinase (GuK) domain. All MAGUKs studied to date localize to regions of cell-cell contact such as tight junctions in epithelial cells and synaptic junctions in neurons and are believed to be involved in the assembly of multi-protein complexes via their protein-protein interaction domains. Three novel, closely related MAGUK proteins were isolated by means of different screening assays, and named MAGI1/BAP1, MAGI2/ S-SCAM /ARIP, and SLIPR/MAGI3. MAGI1 was first identified in mouse as a protein interacting with K-RasB. Three isoforms were identified, and named MAGI1a, MAGI1b, and MAGI1c, of 1139, 1171, and 1374 amino acids respectively. The isolation of a growing number of MAGI1 binding partners, together with the determination of the subcellular and tissue distribution of the different isoforms, are paving the way towards elucidation of its function.
  • Cellular localizationCell junction; cell membrane; tight junction; peripheral membrane protein. Localizes to epithelial cells tight junctions.
  • Database links
  • Alternative names
    • AIP 3 antibody
    • AIP3 antibody
    • Atrophin 1 interacting protein 3 antibody
    • BAI1 associated protein 1 antibody
    • BAIAP 1 antibody
    • BAIAP1 antibody
    • BAP 1 antibody
    • BAP1 antibody
    • Gukmi1 antibody
    • MAGI 1 antibody
    • MAGI1c antibody
    • Membrane associated guanylate kinase inverted 1 antibody
    • membrane associated guanylate kinase WW and PDZ domain containing 1 antibody
    • TNRC 19 antibody
    • TNRC19 antibody
    • Trinucleotide repeat containing gene 19 antibody
    • Trinucleotide repeat containing gene 19 protein antibody
    • WW domain containing protein 3 antibody
    • WWP 3 antibody
    • WWP3 antibody
    see all

Anti-MAGI1 antibody images

  • Anti-MAGI1 antibody (ab37543) at 1/100 dilution + Mouse brain cell lysate

    Predicted band size : 137 kDa
    Observed band size : 137 kDa
    Additional bands at : 50 kDa,60 kDa. We are unsure as to the identity of these extra bands.
  • ab37543, at a 1/50 dilution, staining human hepatocarcinoma tissue by immunohistochemistry, formalin-fixed paraffin-embedded tissue, followed by AEC staining.

References for Anti-MAGI1 antibody (ab37543)

This product has been referenced in:
  • Ridgway LD  et al. MAGI-1 interacts with Slo1 channel proteins and suppresses Slo1 expression on the cell surface. Am J Physiol Cell Physiol 297:C55-65 (2009). Read more (PubMed: 19403801) »

See 1 Publication for this product

Product Wall

Thank you for your patience. Here is the clarifying explanation regarding the immunogen: The immunogenic sequence was chosen based on the mutated sequence in Q96QZ7-3 or 4 and therefore it is absent in other isoforms including isoform 1.  It is i...

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Thank you for contacting us. I heard back from the lab with the following information: ab37543 can potentially recognize isoform 3 or 4. However, I am confused about this reply since the isoforms 3 and 4 (according to the UniProt database entry...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"