The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 130, 160 kDa (predicted molecular weight: 123, 160 kDa).
1/100 - 1/500.
FunctionActs as a scaffolding protein at cell-cell junctions, thereby regulating various cellular and signaling processes. Cooperates with PTEN to modulate the kinase activity of AKT1. Its interaction with PTPRB and tyrosine phosphorylated proteins suggests that it may link receptor tyrosine phosphatase with its substrates at the plasma membrane. In polarized epithelial cells, involved in efficient trafficking of TGFA to the cell surface. Regulates the ability of LPAR2 to activate ERK and RhoA pathways. Regulates the JNK signaling cascade via its interaction with FZD4 and VANGL2.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the MAGUK family. Contains 1 guanylate kinase-like domain. Contains 6 PDZ (DHR) domains. Contains 2 WW domains.
Post-translational modificationsUbiquitinated following interaction with HPV E6 protein, leading to its degradation by the proteasome. Degradation is independent of E6AP ubiquitin ligase complex.
Cellular localizationCell membrane. Cell junction > tight junction. Nucleus. Concentrates in specific sites at the plasma membrane and in the nucleus. In epithelial cells, it localizes at tight junctions.