Overview

  • Product nameAnti-MAGI3 antibody [SLP-32]
    See all MAGI3 primary antibodies
  • Description
    Mouse monoclonal [SLP-32] to MAGI3
  • SpecificityMAGI3 (membrane-associated guanylate cyclase of inverted orientation-3) is a member of the family of membrane-associated guanylate kinases (MAGUKs) that comprise peripheral membrane proteins involved in the formation of specialized cell-cell junctions. MAGI3, as well as other proteins in this family are characterized by three types of protein-protein interaction modules: five PDZ domains, an N-terminal guanylate kinase (GK) domain, and a WW domain. The WW domain, named for two conserved tryptophan residues and first identified as two repeats in mouse YAP65, mediates protein-protein interactions through binding to short proline-rich sequences). MAGI3 and PTEN/MMAC together modulate the kinase activity of AKT/protein kinase B (PKB). MAGI3 positions PTEN/MMAC phosphatase to specific subcellular locations that are involved in the regulation of cell proliferation and survival.
  • Tested applicationsSuitable for: ELISA, WB, ICCmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Synthetic peptide, corresponding to C-term amino acids 421-785 of Rat MAGI3.

  • Positive control
    • Tested with rat brain extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab11509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 160 kDa.
ICC Use at an assay dependent dilution.

Target

  • FunctionActs as a scaffolding protein at cell-cell junctions, thereby regulating various cellular and signaling processes. Cooperates with PTEN to modulate the kinase activity of AKT1. Its interaction with PTPRB and tyrosine phosphorylated proteins suggests that it may link receptor tyrosine phosphatase with its substrates at the plasma membrane. In polarized epithelial cells, involved in efficient trafficking of TGFA to the cell surface. Regulates the ability of LPAR2 to activate ERK and RhoA pathways. Regulates the JNK signaling cascade via its interaction with FZD4 and VANGL2.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesBelongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 6 PDZ (DHR) domains.
    Contains 2 WW domains.
  • Post-translational
    modifications
    Ubiquitinated following interaction with HPV E6 protein, leading to its degradation by the proteasome. Degradation is independent of E6AP ubiquitin ligase complex.
  • Cellular localizationCell membrane. Cell junction > tight junction. Nucleus. Concentrates in specific sites at the plasma membrane and in the nucleus. In epithelial cells, it localizes at tight junctions.
  • Information by UniProt
  • Database links
  • Alternative names
    • dJ730K3.2 antibody
    • MAGI-3 antibody
    • magi3 antibody
    • MAGI3_HUMAN antibody
    • Membrane Associated Guanylate kinase Related 3 antibody
    • Membrane-associated guanylate kinase antibody
    • Membrane-associated guanylate kinase inverted 3 antibody
    • RP4-730K3.1 antibody
    • SLIPR antibody
    • WW and PDZ domain-containing protein 3 antibody
    see all

References for Anti-MAGI3 antibody [SLP-32] (ab11509)

ab11509 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"