Recombinant
RabMAb

Anti-Mago nashi homolog 2 antibody [EPR14037-43] (ab186431)

Overview

  • Product name
    Anti-Mago nashi homolog 2 antibody [EPR14037-43]
  • Description
    Rabbit monoclonal [EPR14037-43] to Mago nashi homolog 2
  • Host species
    Rabbit
  • Specificity
    The immunogen shares 100% homology with both MAGOHB (UniProt Q96A72) and MAGOH (UniProt P61326). We have not performed any tests in order to determine the cross-reactivity but it is possible that ab186431 could cross-react with both MAGOHB and MAGOH. Both proteins have a similar molecular weight of 17 kDa.
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Mago nashi homolog 2 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P61326

  • Positive control
    • HeLa, Jurkat, Raw264.7, PC12 and NIH/3T3 cell lysate. Human brain and Human prostatic hyperplasia tissue. HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab186431 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
IHC-P 1/500 - 1/1000.
ICC/IF 1/250.

Target

Images

  • Immunofluorescent analysis of 4% paraformaldehyde fixed HeLa cells labeling Mago nashi homolog 2 with ab186431 at 1/250 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution. Counterstained with Dapi blue.

  • All lanes :

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 17 kDa
    Observed band size: 17 kDa

  • Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling Mago nashi homolog 2 with ab186431 at 1/1000 dilution. Counter stain Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Mago nashi homolog 2 with ab186431 at 1/1000 dilution. Counter stain Hematoxylin.

  • All lanes :

    Lane 1 : Raw264.7 cell lysate
    Lane 2 : PC12 cell lysate
    Lane 3 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated

    Predicted band size: 17 kDa
    Observed band size: 17 kDa

References

ab186431 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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