Overview

  • Product nameAnti-MAGOH antibodySee all MAGOH primary antibodies ...
  • Description
    Rabbit polyclonal to MAGOH
  • Tested applicationsWB, ICC/IF, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Xenopus laevis, Fruit fly (Drosophila melanogaster), Zebrafish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human MAGOH.

    (Peptide available as ab28608.)

  • Positive control
    • HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate, Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate, A431 (Human epithelial carcinoma cell line) Whole Cell Lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab38768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
IP
IHC-P
  • Application notesIHC-P: Use at a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    ICC/IF: 1/200 (please refer to abreview for further details).
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa). This antibody also detects a band of approximately 37 kDa which we believe to be unreduced complex containing MAGOH.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionComponent of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Remains associated with the mRNA after its export to the cytoplasm and require translation of the mRNA for removal. The heterodimer MAGOH-RBM8A interacts with PYM that function to enhance the translation of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex.
    • Tissue specificityUbiquitous.
    • Sequence similaritiesBelongs to the mago nashi family.
    • Cellular localizationNucleus. Nucleus speckle. Cytoplasm. Detected in granule-like structures in the dendroplasm (By similarity). Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Colocalizes with the core EJC, THOC4, NXF1 and UAP56 in the nucleus and nuclear speckles.
    • Information by UniProt
    • Database links
    • Alternative names
      • Mago nashi homolog proliferation associated (Drosophila) antibody
      • Mago nashi protein homolog antibody
      • magoh antibody
      • MAGOHA antibody
      • MGN_HUMAN antibody
      • Protein mago nashi homolog antibody
      see all

    Anti-MAGOH antibody images

    • MAGOH was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to MAGOH and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

      The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

      Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab38768.

      Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

      Band: 17kDa, non specific bands - 37kDa: We are unsure as to the identity of this extra band;

      MAGOH

    • ab38768 staining MAGOH in HeLa cells (green). Cells were fixed in Paraformaldehyde and counterstained with DAPI (red). Please refer to abreview for further details.

      See Abreview

    • IHC image of MAGOH staining in human cerebral cortex FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab38768, 1µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    • All lanes : Anti-MAGOH antibody (ab38768) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size : 17 kDa
      Observed band size : 17 kDa
      Additional bands at : 26 kDa,37 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 3 minutes

    References for Anti-MAGOH antibody (ab38768)

    This product has been referenced in:
    • Silver DL  et al. The exon junction complex component Magoh controls brain size by regulating neural stem cell division. Nat Neurosci 13:551-8 (2010). WB ; Mouse . Read more (PubMed: 20364144) »

    See 1 Publication for this product

    Product Wall

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I have issued a free of charge replacement with the order number **** for ab10686. To check the status of th...

    Read More
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Paraformaldehyde
    Username

    Dr. Kirk McManus

    Verified customer

    Submitted Mar 29 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"