Recombinant
RabMAb

Anti-MAGOH antibody [EPR10745] (ab170944)

Overview

  • Product name
    Anti-MAGOH antibody [EPR10745]
    See all MAGOH primary antibodies
  • Description
    Rabbit monoclonal [EPR10745] to MAGOH
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human MAGOH. The exact sequence is proprietary.
    Database link: P61326

  • Positive control
    • K562, Raji, HeLa and HL60 cell lysates; Human kidney tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab170944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 17 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Remains associated with the mRNA after its export to the cytoplasm and require translation of the mRNA for removal. The heterodimer MAGOH-RBM8A interacts with PYM that function to enhance the translation of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex.
    • Tissue specificity
      Ubiquitous.
    • Sequence similarities
      Belongs to the mago nashi family.
    • Cellular localization
      Nucleus. Nucleus speckle. Cytoplasm. Detected in granule-like structures in the dendroplasm (By similarity). Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Colocalizes with the core EJC, THOC4, NXF1 and UAP56 in the nucleus and nuclear speckles.
    • Information by UniProt
    • Database links
    • Alternative names
      • Mago nashi homolog proliferation associated (Drosophila) antibody
      • Mago nashi protein homolog antibody
      • magoh antibody
      • MAGOHA antibody
      • MGN_HUMAN antibody
      • Protein mago nashi homolog antibody
      see all

    Images

    • Immunohistochemistry analysis of paraffin-embedded Human kidney tissue labeling MAGOH with ab170944 at 1/50 dilution.

    • All lanes : Anti-MAGOH antibody [EPR10745] (ab170944) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : Raji cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : HL60 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 17 kDa

    References

    ab170944 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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