The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 21 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionComponent of the elastin-associated microfibrils.
Sequence similaritiesBelongs to the MFAP family. Contains 1 SXC (ShKT) domain.
Post-translational modificationsForms intermolecular disulfide bonds either with other MAGP-1 molecules or with other components of the microfibrils. May form transglutaminase cross-links. O-glycosylated.
Cellular localizationSecreted > extracellular space > extracellular matrix.