• Product nameAnti-MAK (phospho Y159) antibody
    See all MAK primary antibodies
  • Description
    Rabbit polyclonal to MAK (phospho Y159)
  • Specificityab92562 detects endogenous levels of MAK only when phosphorylated at tyrosine 159.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from Human MAK around the phosphorylation site of tyrosine 159 (TDYPVS).

  • Positive control
    • HepG2 cells (treated with PMA at 125ng/ml for 30mins)


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab92562 was purified by affinity-chromatography using an epitope-specific phosphopeptide. The antibody against the non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab92562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesELISA: 1/40000.
    WB: 1/500 - 1/1000. Predicted molecular weight: 71 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionEssential for the regulation of ciliary length and required for the long-term survival of photoreceptors (By similarity). Phosphorylates FZR1 in a cell cycle-dependent manner. Plays a role in the transcriptional coactivation of AR. Could play an important function in spermatogenesis. May play a role in chromosomal stability in prostate cancer cells.
    • Tissue specificityExpressed in prostate cancer cell lines at generally higher levels than in normal prostate epithelial cell lines. Isoform 1 is expressed in kidney, testis, lung, trachea, and retina. Isoform 2 is retina-specific where it is expressed in rod and cone photoreceptors.
    • Involvement in diseaseDefects in MAK are the cause of retinitis pigmentosa type 62 (RP62) [MIM:614181]. RP62 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
    • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      Autophosphorylated. Phosphorylated on serine and threonine residues.
    • Cellular localizationNucleus. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Midbody. Cell projection > cilium > photoreceptor outer segment. Photoreceptor inner segment. Localized in both the connecting cilia and the outer segment axonemes (By similarity). Localized uniformly in nuclei during interphase, to the mitotic spindle and centrosomes during metaphase and anaphase, and also to midbody at anaphase until telophase.
    • Information by UniProt
    • Database links
    • Alternative names
      • intestinal cell kinase antibody
      • dJ417M14.2 antibody
      • fj04c02 antibody
      • Ick antibody
      • Mak antibody
      • MAK_HUMAN antibody
      • Male germ cell associated kinase antibody
      • Male germ cell-associated kinase antibody
      • male germ cell-associated kinase retinal-enriched isoform antibody
      • OTTHUMP00000016025 antibody
      • Rck antibody
      • RP62 antibody
      • Serine/threonine protein kinase MAK antibody
      • Serine/threonine-protein kinase MAK antibody
      • testicular secretory protein Li 28 antibody
      • wu:fj04c02 antibody
      • zgc:56603 antibody
      see all

    Anti-MAK (phospho Y159) antibody images

    • All lanes : Anti-MAK (phospho Y159) antibody (ab92562) at 1/500 dilution

      Lane 1 : HepG2 cells, treated with PMA (125ng/ml, 30mins)
      Lane 2 : HepG2 cells, treated with PMA (125ng/ml, 30mins) with immunising peptide at 10 µg

      Lysates/proteins at 30 µg per lane.

      Predicted band size : 71 kDa

    References for Anti-MAK (phospho Y159) antibody (ab92562)

    ab92562 has not yet been referenced specifically in any publications.

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