Overview

  • Product name
  • Description
    Rabbit polyclonal to MAK16
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Orangutan, Bat
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 250-300 of Human MAK16 (NP_115898.2)

  • Positive control
    • HeLa 293T whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab74224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 35 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Cellular localization
    Nuclear; nucleolus
  • Database links
  • Alternative names
    • MAK16 homolog (S. cerevisiae) antibody
    • MAK16 homolog antibody
    • MAK16L antibody
    • NNP78 antibody
    • protein MAK16 homolog antibody
    • Protein RBM13 antibody
    • RBM13 antibody
    • RNA binding motif protein 13 antibody
    • RNA binding protein antibody
    see all

Images

  • All lanes : Anti-MAK16 antibody (ab74224) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 35 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 51 kDa. We are unsure as to the identity of these extra bands.
  • 1mg HeLa whole cell lysate was immunoprecipitated using ab74224 at 3µg/mg lysate (lane 1) or a control IgG (lane 2). 20% of the immunoprecipitate was used for Western blotting. Bands were identified using ab74224 at 0.1µg/ml and detected by chemiluminescence with an exposure time of 10 seconds.

References

ab74224 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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