1mg HeLa whole cell lysate was immunoprecipitated using ab74224 at 3µg/mg lysate (lane 1) or a control IgG (lane 2). 20% of the immunoprecipitate was used for Western blotting. Bands were identified using ab74224 at 0.1µg/ml and detected by chemiluminescence with an exposure time of 10 seconds.
References for Anti-MAK16 antibody (ab74224)
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