IHC image of ab15418 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab15418, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-MAL antibody (ab15418)
This product has been referenced in:
Carmosino M et al. MAL/VIP17, a new player in the regulation of NKCC2 in the kidney. Mol Biol Cell21:3985-97 (2010).
Read more (PubMed: 20861303) »
Kamsteeg EJ et al. MAL decreases the internalization of the aquaporin-2 water channel. Proc Natl Acad Sci U S A104:16696-701 (2007).
Read more (PubMed: 17940053) »