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Abcam’s anti-Malaria Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG and IgM class antibodies against Malaria in Human serum and plasma.
A 96-well plate has been precoated with Malaria antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG and anti-Human IgM conjugate is added to the wells, which binds to the immobilized Malaria antigens. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Malaria IgG or Malaria IgM sample captured in plate.
|Components||Identifier||1 x 96 tests|
|20X Washing Solution||White cap||1 x 50ml|
|Cover Foil||1 unit|
|IgG Sample Diluent||1 x 100ml|
|Malaria Anti-IgG and Anti-IgM HRP Conjugate||1 x 20ml|
|Malaria Coated Microplate (12 x 8 wells)||1 unit|
|Malaria Cut-off Control||1 x 3ml|
|Malaria Negative Control||1 x 2ml|
|Malaria Positive Control||1 x 2ml|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|TMB Substrate Solution||Yellow cap||1 x 15ml|
Our Abpromise guarantee covers the use of ab178649 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent concentration.|
ab178649 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"