In Abcam's Malate Dehydrogenase Activity Assay kit (Colorimetric) (ab183305), malate dehydrogenase (MDH) reacts with malate to form an intermediate, which in turn reacts with MDH Developer to form a colored product with strong absorbance at 450 nm. The assay is simple, sensitive and can detect less than 0.5 mU of MDH activity in various sample types.
This assay detects both isoforms of MDH: MDH1 and MDH2. If you want to detect enzymatic activity of each specific isoform, please consider MDH1 Activity Assay (ab200009) or MDH2 activity Assay (ab119693).
Malate Dehydrogenase (MDH) (EC 188.8.131.52) is an important enzyme which reversibly converts L-malate into oxaloacetate in the presence of NAD. In eukaryotic cells, malate dehydrogenase has 2 isoforms: MDH1 and MDH2. MDH1 is cytosolic & participates in the malateaspartate shuttle, which transports malate into mitochondria for utilization in ATP generation whereas MDH2 is a mitochondrial enzyme and part of the citric acid cycle. MDH activity is increased in some neurodegenerative diseases such as Alzheimer’s disease, and abnormal MDH activity in serum can serve as a diagnostic tool for severe liver damage (e.g. Hepatocellular carcinoma).
|MDH Assay Buffer||1 x 20ml|
|MDH Developer||1 vial|
|MDH Enzyme Mix||1 vial|
|MDH Positive Control||1 vial|
|MDH Substrate||1 vial|
|NADH Standard (Lyophilized)||Yellow||1 vial|
Our Abpromise guarantee covers the use of ab183305 in the following tested applications.
|Functional Studies||Use at an assay dependent concentration.|
ab183305 has not yet been referenced specifically in any publications.