Malate Dehydrogenase Activity Assay Kit (Colorimetric) (ab183305)

Overview

  • Product name
    Malate Dehydrogenase Activity Assay Kit (Colorimetric)
  • Detection method
    Colorimetric
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Assay type
    Quantitative
  • Sensitivity
    < 0.5 mU/well
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Rat, Human, Saccharomyces cerevisiae
  • Product overview

    In Abcam's Malate Dehydrogenase Activity Assay kit (Colorimetric) (ab183305), malate dehydrogenase (MDH) reacts with malate to form an intermediate, which in turn reacts with MDH Developer to form a colored product with strong absorbance at 450 nm. The assay is simple, sensitive and can detect less than 0.5 mU of MDH activity in various sample types.


    This assay detects both isoforms of MDH: MDH1 and MDH2. If you want to detect enzymatic activity of each specific isoform, please consider MDH1 Activity Assay (ab200009) or MDH2 activity Assay (ab119693).

  • Notes

    Malate Dehydrogenase (MDH) (EC 1.1.1.37) is an important enzyme which reversibly converts L-malate into oxaloacetate in the presence of NAD. In eukaryotic cells, malate dehydrogenase has 2 isoforms: MDH1 and MDH2. MDH1 is cytosolic & participates in the malateaspartate shuttle, which transports malate into mitochondria for utilization in ATP generation whereas MDH2 is a mitochondrial enzyme and part of the citric acid cycle. MDH activity is increased in some neurodegenerative diseases such as Alzheimer’s disease, and abnormal MDH activity in serum can serve as a diagnostic tool for severe liver damage (e.g. Hepatocellular carcinoma).

  • Tested applications
    Suitable for: Functional Studiesmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab183305 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • Rat muscle extract (0.8 μg)

    Jurkat cell lysate (0.6 μg)

    Yeast mitochondria (1.2 μg)

    MDH positive control.

Protocols

References

ab183305 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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