- Datasheet
- Specific References (10)
- Protocols
Overview
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Product nameAnti-Malondialdehyde antibody
See all Malondialdehyde primary antibodies -
DescriptionRabbit polyclonal to Malondialdehyde
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Host speciesRabbit
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SpecificityThis antibody specifically binds to Malondialdehyde LDL and other Malondialdehyde modified proteins.
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Tested applicationsSuitable for: ELISA, WB, Conjugationmore details
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Immunogen
Chemical/ Small Molecule MDA modified protein
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 0.2% PBS, 0.0292% EDTA, 0.435% Sodium chloride, 30% Glycerol -
Concentration information loading... -
Purification notesThis antibody was purified by MDA modified protein Sepharose affinity column.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab27642 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | 1/5000 - 1/40000. | |
| WB | 1/5000 - 1/40000. | |
| Conjugation | Use at an assay dependent dilution. |
Target
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RelevanceMalondialdehyde (MDA) is a natural product formed in all mammalian cells as a product of lipid peroxidation. MDA is a highly reactive three carbon dialdehyde produced as a byproduct of polyunsaturated fatty acid peroxidation and arachidonic acid metabolism. MDA readily combines with several functional groups on molecules including proteins, lipoproteins, and DNA. It reacts with DNA to form adducts to deoxyguanosine and deoxyadenosine. The major adduct to DNA is a pyrimidopurinone called M1G which appears to be a major endogenous DNA adduct in human beings that may contribute significantly to cancer linked to lifestyle and dietary factors. MDA modified proteins may show altered physico chemical behavior and antigenicity. MDA is toxic and has been implicated in aging mutagenesis, carcinogenesis, diabetic nephropathy and radiation damage. Increased expression of MDA has been reported in the brains of Alzheimer's patients. Antibodies to MDA will help to visualize the MDA adducts.
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Alternative names
- 1133 Tetramethoxypropane antibody
- MDA antibody
Images
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Western blot - Anti-Malondialdehyde antibody (ab27642)This image is courtesy of an Abreview submitted by Chris MattisonAll lanes : Anti-Malondialdehyde antibody (ab27642) at 1/1000 dilution (in PBST for 18 hours at 4°C)
Lane 1 : Whole tissue lysate of raw cashew nuts
Lane 2 : Whole tissue lysate of roasted cashew nuts
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : An HRP-conjugated goat anti-rabbit polyclonal
Developed using the ECL technique.
Observed band size: 10-12 kDa (why is the actual band size different from the predicted?)
Exposure time: 2 minutes
Blocking Step: 2% Milk for 30 minutes at 25°C
Protocols
References
This product has been referenced in:
- Carnuta MG et al. Dysfunctional high-density lipoproteins have distinct composition, diminished anti-inflammatory potential and discriminate acute coronary syndrome from stable coronary artery disease patients. Sci Rep 7:7295 (2017). Read more (PubMed: 28779156) »
- Huang YH et al. Postnatal High-Fat Diet Increases Liver Steatosis and Apoptosis Threatened by Prenatal Dexamethasone through the Oxidative Effect. Int J Mol Sci 17:369 (2016). WB . Read more (PubMed: 26978357) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"