Overview

  • Product nameAnti-Malondialdehyde antibody
    See all Malondialdehyde primary antibodies
  • Description
    Rabbit polyclonal to Malondialdehyde
  • SpecificityThis antibody specifically binds to Malondialdehyde LDL and other Malondialdehyde modified proteins.
  • Tested applicationsSuitable for: ELISA, WB, Conjugationmore details
  • Immunogen

    Chemical/ Small Molecule MDA modified protein

Properties

Applications

Our Abpromise guarantee covers the use of ab27642 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000 - 1/40000.
WB 1/5000 - 1/40000.
Conjugation Use at an assay dependent dilution.

Target

  • RelevanceMalondialdehyde (MDA) is a natural product formed in all mammalian cells as a product of lipid peroxidation. MDA is a highly reactive three carbon dialdehyde produced as a byproduct of polyunsaturated fatty acid peroxidation and arachidonic acid metabolism. MDA readily combines with several functional groups on molecules including proteins, lipoproteins, and DNA. It reacts with DNA to form adducts to deoxyguanosine and deoxyadenosine. The major adduct to DNA is a pyrimidopurinone called M1G which appears to be a major endogenous DNA adduct in human beings that may contribute significantly to cancer linked to lifestyle and dietary factors. MDA modified proteins may show altered physico chemical behavior and antigenicity. MDA is toxic and has been implicated in aging mutagenesis, carcinogenesis, diabetic nephropathy and radiation damage. Increased expression of MDA has been reported in the brains of Alzheimer's patients. Antibodies to MDA will help to visualize the MDA adducts.
  • Alternative names
    • 1133 Tetramethoxypropane antibody
    • MDA antibody

Anti-Malondialdehyde antibody images

  • All lanes : Anti-Malondialdehyde antibody (ab27642) at 1/1000 dilution (in PBST for 18 hours at 4°C)

    Lane 1 : Whole tissue lysate of raw cashew nuts
    Lane 2 : Whole tissue lysate of roasted cashew nuts

    Lysates/proteins at 50 µg per lane.

    Secondary
    An HRP-conjugated goat anti-rabbit polyclonal
    Developed using the ECL technique

    Observed band size : 10-12 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

    This image is courtesy of an Abreview submitted by Chris Mattison

References for Anti-Malondialdehyde antibody (ab27642)

This product has been referenced in:
  • Yamada T  et al. Nitrosative modifications of the Ca2+ release complex and actin underlie arthritis-induced muscle weakness. Ann Rheum Dis N/A:N/A (2014). WB . Read more (PubMed: 24854355) »
  • Cheng AJ  et al. Antioxidant treatments do not improve force recovery after fatiguing stimulation of mouse skeletal muscle fibres. J Physiol N/A:N/A (2014). Read more (PubMed: 25398530) »

See all 5 Publications for this product

Product Wall

Thank you for your inquiry. As this is a small molecule, there is no species reactivity associated with it. We expect the antibody to recognize Malondialdehyde regardless of the source. I hope this information is helpful. Please do not hesitate...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Mouse penile tissue)
Loading amount 60 µg
Specification Mouse penile tissue
Gel Running Conditions Reduced Denaturing (4-20% mini gel)
Blocking step Starting Block T20 (PBS) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Miss. gwen Lagoda

Verified customer

Submitted Feb 13 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Human Penile Tissue)
Loading amount 60 µg
Specification Human Penile Tissue
Gel Running Conditions Reduced Denaturing (4-20% mini gel)
Blocking step (agent) for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Miss. gwen Lagoda

Verified customer

Submitted Jan 30 2012

Thank you for contacting us.

I would suggest analyzing the entire lane. The MDA-modifications and their extent of it will vary from sample to sample as well as from species to species.

It might very well be that in cashews there is...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Cashew nuts (Anacardiaceae) Tissue lysate - whole (whole nut)
Loading amount 50 µg
Specification whole nut
Treatment roasted
Gel Running Conditions Reduced Denaturing (4-20)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Mr. Chris Mattison

Verified customer

Submitted Oct 18 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"