The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/5000 - 1/40000.
1/5000 - 1/40000.
Use at an assay dependent dilution.
Malondialdehyde (MDA) is a natural product formed in all mammalian cells as a product of lipid peroxidation. MDA is a highly reactive three carbon dialdehyde produced as a byproduct of polyunsaturated fatty acid peroxidation and arachidonic acid metabolism. MDA readily combines with several functional groups on molecules including proteins, lipoproteins, and DNA. It reacts with DNA to form adducts to deoxyguanosine and deoxyadenosine. The major adduct to DNA is a pyrimidopurinone called M1G which appears to be a major endogenous DNA adduct in human beings that may contribute significantly to cancer linked to lifestyle and dietary factors.
MDA modified proteins may show altered physico chemical behavior and antigenicity. MDA is toxic and has been implicated in aging mutagenesis, carcinogenesis, diabetic nephropathy and radiation damage. Increased expression of MDA has been reported in the brains of Alzheimer's patients. Antibodies to MDA will help to visualize the MDA adducts.
1133 Tetramethoxypropane antibody
Western blot - Anti-Malondialdehyde antibody (ab27642)
All lanes : Anti-Malondialdehyde antibody (ab27642) at 1/1000 dilution (in PBST for 18 hours at 4°C)
Lane 1 : Whole tissue lysate of raw cashew nuts Lane 2 : Whole tissue lysate of roasted cashew nuts
Lysates/proteins at 50 µg per lane.
Secondary An HRP-conjugated goat anti-rabbit polyclonal Developed using the ECL technique
Carnuta MG et al. Dysfunctional high-density lipoproteins have distinct composition, diminished anti-inflammatory potential and discriminate acute coronary syndrome from stable coronary artery disease patients. Sci Rep7:7295 (2017).
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Huang YH et al. Postnatal High-Fat Diet Increases Liver Steatosis and Apoptosis Threatened by Prenatal Dexamethasone through the Oxidative Effect. Int J Mol Sci17:369 (2016).
Read more (PubMed: 26978357) »