Overview

  • Product nameAnti-Malondialdehyde antibody
    See all Malondialdehyde primary antibodies
  • Description
    Rabbit polyclonal to Malondialdehyde
  • SpecificityUsing a conjugate Malondialdehyde-PC, antibody specificity was performed with an ELISA test by competition experiments with the following compounds: Malondialdehyde-PC with a Cross-reactivity ratio = 1*. 4-hydroxynonenal-PC with a Cross-reactivity ratio = 1/>50,000. Acrolein-PC with a Cross-reactivity ratio = 1/>50,000. Formaldehyde-PC with a Cross-reactivity ratio = 1/>50,000. Azelaic Acid-PC with a Cross-reactivity ratio = 1/>50,000. * Malondialdehyde-PC concentration/conjugated close related structures concentration at half displacement PC = protein carrier
  • Tested applicationsSuitable for: WB, IHC-Fr, ELISA, IHC-Pmore details
  • Immunogen

    Chemical/ Small Molecule: Synthetic Malondialdehyde conjugated to bovine serum albumin (BSA)(available as ab126288).

Properties

Applications

Our Abpromise guarantee covers the use of ab6463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Recommended blocking agent is horse serum.
IHC-Fr 1/1000 - 1/5000.
ELISA Use at an assay dependent concentration.
IHC-P 1/1000 - 1/5000.

Target

  • RelevanceMalondialdehyde (MDA) is a natural product formed in all mammalian cells as a product of lipid peroxidation. MDA is a highly reactive three carbon dialdehyde produced as a byproduct of polyunsaturated fatty acid peroxidation and arachidonic acid metabolism. MDA readily combines with several functional groups on molecules including proteins, lipoproteins, and DNA. It reacts with DNA to form adducts to deoxyguanosine and deoxyadenosine. The major adduct to DNA is a pyrimidopurinone called M1G which appears to be a major endogenous DNA adduct in human beings that may contribute significantly to cancer linked to lifestyle and dietary factors. MDA modified proteins may show altered physico chemical behavior and antigenicity. MDA is toxic and has been implicated in aging mutagenesis, carcinogenesis, diabetic nephropathy and radiation damage. Increased expression of MDA has been reported in the brains of Alzheimer's patients. Antibodies to MDA will help to visualize the MDA adducts.
  • Alternative names
    • 1133 Tetramethoxypropane antibody
    • MDA antibody

Anti-Malondialdehyde antibody images

  • ab6463 staining Malondialdehyde in human rectal cancer tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer pH 6.0. Samples were then permeabilized, blocked using 5% serum for 1 hour at 4°C then incubated with ab6463 at a 1/500 dilution for 24 hours at 4°C. The secondary used was a biotin conjugated goat anti-rabbit polyclonal, used at a 1/500 dilution.

    See Abreview

  • Immunohistochemical analysis of rat hippocampus tissue, sub-regions CA1, CA3 and DG, staining Malondialdehyde with ab6463. Rats were treated with saline (control) or multiple doses of methamphetatime.

    Sections were fixed in paraformaldehyde and blocked with 10% normal horse serum for 2 hours at room temperature. Samples were incubated with primary antibody (1/200) overnight at 4°C. Staining was detected using DAB.

References for Anti-Malondialdehyde antibody (ab6463)

This product has been referenced in:
  • Fiuza-Luces C  et al. Muscle Signaling in Exercise Intolerance: Insights from the McArdle Mouse Model. Med Sci Sports Exerc N/A:N/A (2016). WB . Read more (PubMed: 27031745) »
  • Rantakari P  et al. Stabilin-1 expression defines a subset of macrophages that mediate tissue homeostasis and prevent fibrosis in chronic liver injury. Proc Natl Acad Sci U S A 113:9298-303 (2016). IHC-P, ICC/IF ; Mouse . Read more (PubMed: 27474165) »

See all 16 Publications for this product

Product Wall

Thank you for your patience.

The product is now available for sale and in our catalog as ab126288 - Malondialdehyde protein (BSA Conjugate)

http://www.abcam.com/index.html?datasheet=126288

I hope this information is helpf...

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Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 23°C
Sample Human Cell (Fetal Retinal Pigment Epithelium)
Specification Fetal Retinal Pigment Epithelium
Permeabilization Yes - 0.15% Triton X-100
Fixative Paraformaldehyde
Username

Dr. Jason Miller

Verified customer

Submitted Jan 02 2015

Thank you for your inquiry.

The image on our datasheet was submitted by a customer as part of an Abreview. http://www.abcam.com/Malondialdehyde-antibody-ab6463/reviews/25826

Unfortunately it's difficult to say exactly what type of ce...

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Ab6463 has been tested in and is guaranteed to work in WB, IHC-Fr, ELISA and IHC-P. When using in IHC-P we recommend heat mediated antigen retrieval using citrate buffer pH 6.0, generally heating between 10-20 minutes is adequate. I have included Abca...

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Muchas gracias por tomarte la molestia de mandarnos el cuestionario relleno y las imágenes.
Atendiendo al protocolo seguido, me gustaría hacer un par de sugerencias para tratar de optimizar el resultado.
- Es muy posible que el motivo por el ...

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Gracias por ponerte en contacto con nosotros y alertarnos del problema experimentado con nuestro producto. Cualquier comentario o queja procedente de nuestros clientes es muy importante para mejorar y monitorizar la calidad de nuestros productos.
...

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Thank you for your reply.



This product is covered by our Abpromise guarantee. Should you experience and issues when using this according to the datasheet. We are happy provide scientific support, replacement or refund. More infor...

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Thank you for contacting us.

This product is directed against a small molecule and as such is not species specific. We do guarantee it to work in all species samples whereMalondialdehyde is present, in the tested applications: WB, IHC-Fr, ...

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Thank you for contacting Abcam regarding ab6463.


I can confirm that liver tissue sections would be a suitable positive control to use with this antibody.


I hope this information is helpful. Please do not hesitate to contact ...

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Thank you for your reply.


All available references can be found on the Specific References tab on the online datasheet. I did not see a citation specifically for WB, but I have not read through all of the papers.


I hope ...

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1-10 of 24 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"