Anti-Malondialdehyde antibody (FITC) (ab27615)

Overview

  • Product name
    Anti-Malondialdehyde antibody (FITC)
    See all Malondialdehyde primary antibodies
  • Description
    Goat polyclonal to Malondialdehyde (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    This antibody specifically binds to Malondialdehyde.
  • Tested applications
    Suitable for: ICC/IFmore details
  • Immunogen

    Chemical/ Small Molecule MDA modified protein

  • General notes

    Molar FITC/ Protein ratio is 3.1

Properties

Applications

Our Abpromise guarantee covers the use of ab27615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.

Target

  • Relevance
    Malondialdehyde (MDA) is a natural product formed in all mammalian cells as a product of lipid peroxidation. MDA is a highly reactive three carbon dialdehyde produced as a byproduct of polyunsaturated fatty acid peroxidation and arachidonic acid metabolism. MDA readily combines with several functional groups on molecules including proteins, lipoproteins, and DNA. It reacts with DNA to form adducts to deoxyguanosine and deoxyadenosine. The major adduct to DNA is a pyrimidopurinone called M1G which appears to be a major endogenous DNA adduct in human beings that may contribute significantly to cancer linked to lifestyle and dietary factors. MDA modified proteins may show altered physico chemical behavior and antigenicity. MDA is toxic and has been implicated in aging mutagenesis, carcinogenesis, diabetic nephropathy and radiation damage. Increased expression of MDA has been reported in the brains of Alzheimer's patients. Antibodies to MDA will help to visualize the MDA adducts.
  • Alternative names
    • 1133 Tetramethoxypropane antibody
    • MDA antibody

References

ab27615 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 21°C
Antigen retrieval step
None
Sample
Rat Tissue sections (Rat Forebrain)
Specification
Rat Forebrain
Permeabilization
No
Fixative
Paraformaldehyde
Username

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Verified customer

Submitted Oct 23 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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