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Maltose and Glucose Assay Kit (ab65335) can measure glucose or maltose levels in a variety of biological samples (e.g. serum, plasma, body fluids, food, growth medium, etc.). The assay is based on the ability of glucose oxidase to specifically oxidize free glucose, producing a product that interacts with the glucose probe. The amount of product can be either colorimetric (spectrophotometry at OD= 570 nm) or fluorometric (Ex/Em = 535/587 nm) method.
This kit can detect 10 pmol to 10 nmol glucose per assay. It can also be used for isomaltose detection.
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Glucose (C6H12O6; FW: 180.16) and Maltose (C12H22O11; FW: 342.3) are the main fuel sources to generate the universal energy molecule ATP. Maltose is the major disaccharide that generated from hydrolysis of starch in food. Maltose contains two glucose units joined by a α-1,4-glycosidic linkage, which can be easily converted to two glucoses by α-D-glucosidase.
|alpha-D-Glucosidase (Lyophilized)||Blue||1 vial|
|Assay Buffer||WM||1 x 25ml|
|Glucose Enzyme Mix (lyophilized)||Green||1 vial|
|Glucose Probe||Red||1 x 0.2ml|
|Maltose Standard (100 nmol/µl)||Yellow||1 x 100µl|
Our Abpromise guarantee covers the use of ab65335 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
ab65335 has not yet been referenced specifically in any publications.