Overview

  • Product nameMaltose Assay Kit
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeQuantitative
  • Assay time
    1h 00m
  • Product overview

    Abcam's Maltose Assay Kit provides a fast, easy and sensitive method for quantifying maltose in various biological samples (e.g. serum, plasma, body fluids, food, growth medium, etc.).
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Maltose (C12H22O11; FW: 342.3), one of the main fuel sources to generate the universal energy molecule ATP, is the major disaccharide that is generated from hydrolysis of starch in food. Maltose contains two glucose units joined by a a-1,4-glycosidic linkage, which can be easily converted to two glucoses by a-D-glucosidase. The generated glucose can be specifically oxidized to produce a product that interacts with the probe to generate color and fluorescence. Thus, maltose can be determined by either colorimetric (spectrophotometry at ? = 570 nm) or fluorometric (Ex/Em = 535/587 nm) methods.

  • Tested applicationsSuitable for: Functional Studiesmore details

Properties

  • Storage instructionsStore at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    a-D-Glucosidase (Lyophilized) Blue 1 vial
    Enzyme Mix (Lyophilized) Green 1 vial
    Maltose Assay Buffer WM 1 x 25ml
    Maltose Probe in DMSO Red 1 x 200µl
    Maltose Standard (100 nmol/µl) Yellow 1 x 100µl
  • Research areas
  • RelevanceMaltose (C12H22O11; FW: 342.3), one of the main fuel sources to generate the universal energy molecule ATP, is the major disaccharide that is generated from hydrolysis of starch in food.
  • Alternative names
    • 4 O alpha D Glucopyranosyl D glucose
    • Malt sugar

Applications

Our Abpromise guarantee covers the use of ab83388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Maltose Assay Kit images

  • Maltose Standard Curve. Assays were performed following kit instructions. Open circle is maltose with alpha-D-Glucosidase. Solid square is maltose without alpha-D-Glucosidase. Triangle is free glucose (divided by 2).

Protocols

References for Maltose Assay Kit (ab83388)

This product has been referenced in:
  • Axelsson E  et al. The genomic signature of dog domestication reveals adaptation to a starch-rich diet. Nature N/A:N/A (2013). Functional Studies . Read more (PubMed: 23354050) »

See 1 Publication for this product

Product Wall

Extraction of proteins is not necessary, as kit ab83388 measures maltose. Samples in ethanol are not compatible as the enzymes in the kit work in aqueous solution.

Tissue and cell samples can be homogenized in 2-3 volumes of the Maltose Assay ...

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1) Malate Assay Kit (ab83391): detects product formed.
Step 1: Malate in sample + oxidation = product formed.
Step 2: Product formed + probe = color generation.

2) Maltose Assay Kit (ab83388): detects product formed.
Step 1: Maltos...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"