Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab21144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 44 kDa. Dilution optimised using Chromogenic detection.
ELISA 1/10000.

Target

  • Relevance
    Epitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. The insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product. Cleavage by factor Xa separates MBP from its partner protein.
  • Cellular localization
    Periplasm
  • Alternative names
    • ECK4026 antibody
    • JW3994 antibody
    • Mal E antibody
    • MalE antibody
    • malJ antibody
    • Maltodextrin binding protein antibody
    • Maltose ABC transporter periplasmic protein antibody
    • Maltose binding periplasmic protein antibody
    • MMBP antibody
    • Periplasmic maltose binding protein antibody
    see all

Images

  • Anti-Maltose Binding Protein antibody (ab21144) at 1/5000 dilution

    Secondary
    Anti-Rabbit antibody (Sigma A0545) at 1/16000 dilution
    Developed using the ECL technique

    Observed band size : 47.5 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

References

This product has been referenced in:
  • Braun J  et al. Rapid identification of regulatory microRNAs by miTRAP (miRNA trapping by RNA in vitro affinity purification). Nucleic Acids Res 42:e66 (2014). Read more (PubMed: 24510096) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Hela cell)
Specification
Hela cell
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.03% Triton X 100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Abcam user community

Verified customer

Submitted Jan 14 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Saccharomyces cerevisiae Recombinant protein (Recombinant yeast SUMO protein)
Specification
Recombinant yeast SUMO protein
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Type
Direct
Username

Abcam user community

Verified customer

Submitted May 12 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Escherichia coli Recombinant protein (BL21(DE3))
Loading amount
1 µg
Specification
BL21(DE3)
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Nov 03 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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