Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Tris pH7, 50% glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
WB 1/2000.

Target

  • Relevance
    Epitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. The insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product. Cleavage by factor Xa separates MBP from its partner protein.
  • Cellular localization
    Periplasm
  • Alternative names
    • ECK4026 antibody
    • JW3994 antibody
    • Mal E antibody
    • MalE antibody
    • malJ antibody
    • Maltodextrin binding protein antibody
    • Maltose ABC transporter periplasmic protein antibody
    • Maltose binding periplasmic protein antibody
    • MMBP antibody
    • Periplasmic maltose binding protein antibody
    see all

Anti-Maltose Binding Protein antibody images

  • Cell division in a fixed Drosophila melanogaster embryo. The TACC protein is identified in immunofluorescence using the Rabbit polyclonal to MBP which recognises a TACC-MBP fusion.

References for Anti-Maltose Binding Protein antibody (ab9084)

This product has been referenced in:
  • Sánchez H  et al. Architectural plasticity of human BRCA2-RAD51 complexes in DNA break repair. Nucleic Acids Res 45:4507-4518 (2017). Read more (PubMed: 28168276) »
  • Gimenez-Ibanez S  et al. The bacterial effector HopX1 targets JAZ transcriptional repressors to activate jasmonate signaling and promote infection in Arabidopsis. PLoS Biol 12:e1001792 (2014). WB . Read more (PubMed: 24558350) »

See all 7 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Escherichia coli Purified protein (BL21(DE3))
Loading amount
0.5 µg
Specification
BL21(DE3)
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Apr 27 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Escherichia coli Cell lysate - whole cell (Mach1 (E.coil strain,Invitrogen))
Loading amount
0.05 µg
Specification
Mach1 (E.coil strain,Invitrogen)
Treatment
1mg/ml Lysozyme
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Dec 24 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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